Human immunoglobulin g adsorption onto immobilized dye epoxy chitosan/alginate microspheres: mathematical modeling using artificial neural networks

• Grant number: 14/23893-3
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2016
• End date: February 28, 2018
• Field of knowledge: Engineering - Chemical Engineering - Industrial Operations and Equipment for Chemical Engineering
• Principal Investigator: Igor Tadeu Lazzarotto Bresolin
• Grantee: Igor Tadeu Lazzarotto Bresolin
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: Ivanildo José da Silva Junior ; Jones Erni Schmitz

Abstract

Human immunoglobulin G (IgG) in an antibody used for several therapeutic indications. As high concentration and purity are required, selective adsorption techniques, such as affinity chromatography, are recommended for recovery and purification purposes. Thus, this research project aims to evaluate human IgG adsorption in non-conventional affinity-based adsorbents synthetized of chitosan and alginate with Reactive Green, Reactive Blue and Cibacron Blue dyes immobilized as ligands. The adsorption will be evaluated under different experimental conditions, by varying the pH between 6.0 and 8.5 and the temperature between 4 and 37o C. The adsorption data modelling will be performed by using artificial neural networks and compared to classic non-linear methods for Langmuir, Langmuir-Freundlich and Temkin parameters adjusting. Adsorption thermodynamic parameters, such as Gibbs free energy (DG0), enthalpy (DH0) and entropy (DS0) will be estimated via artificial neural networks and van't Hoff and Gibbs-Helmholtz equations. Thus, it will possible to obtain the necessary data to define the optimum adsorption condition aiming to improve the use of this technique in downstream processing of human IgG. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORENO PASSARO, ANA CAROLINA; MOZETIC, TAINA MAIA; SCHMITZ, JONES ERNI; DA SILVA JR, IVANILDO JOSE; MARTINS, TIAGO DIAS; LAZZAROTTO BRESOLIN, IGOR TADEU. Human Immunoglobulin G Adsorption in Epoxy Chitosan/Alginate Adsorbents: Evaluation of Isotherms by Artificial Neural Networks. CHEMICAL PRODUCT AND PROCESS MODELING, v. 14, n. 4, DEC 2019. (16/18005-7, 14/23893-3)