Biomechanical and biomolecular study of therapeutic methods for bone regeneration

• Grant number: 14/26729-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2016
• End date: October 31, 2018
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: William Dias Belangero
• Grantee: William Dias Belangero
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Ana Amélia Rodrigues ; Jose Ricardo Lenzi Mariolani

Abstract

Five to 10% of the bone fractures do not regenerate properly, resulting indelayed union or nonunion and causing physical disability or incapacity of the affectedperson. This fact highlights the importance of studying therapeutic methods foraccelerating bone healing like stimulation by low-intensity pulsed ultrasound andelectromagnetic field. The study of biomechanical and biomolecular aspects of thesemethods will be developed in two steps: in vivo detection of expression and activationof FAK, ERK-1/2, Akt e IRS-1 from rat tibias (intact and with an artificial defect)stimulated by the cited therapeutic methods and in vivo biomechanical evaluation, bymeans of a specially developed intentation test and a tension test, of the boneconsolidation within rat bone defects stimulated by the cited methods. We intend toexamine the correlation between the proteins and the bone strength and, based on thefindings, to determine the advantages and disadvantages of each stimulation method,targeting their clinical application. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LENZI MARIOLANI, JOSE RICARDO; SARAIVA, JOAO FERNANDO; BATISTA, NILZA ALZIRA; BELANGERO, WILLIAM DIAS. Punch shear test for quantitative evaluation of bone defect repair in Wistar rat tibia. INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED, v. 52, n. 3, p. S13-S17, JUL 2021. (14/26729-0)

BUARQUE DE GUSMAO, CARLOS VINICIUS; BATISTA, NILZA ALZIRA; VIDOTTO LEMES, VALERIA TROMBINI; MAIA NETO, WILSON LEITE; DE FARIA, LIDIA DORNELAS; ALVES, JOSE MARCOS; BELANGERO, WILLIAM DIAS. EFFECT OF LOW-INTENSITY PULSED ULTRASOUND STIMULATION, EXTRACORPOREAL SHOCKWAVES AND RADIAL PRESSURE WAVES ON AKT, BMP-2, ERK-2, FAK AND TGF-beta 1 DURING BONE HEALING IN RAT TIBIAL DEFECTS. ULTRASOUND IN MEDICINE AND BIOLOGY, v. 45, n. 8, p. 2140-2161, AUG 2019. (14/26729-0)