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The effects of the Ethyl-2-Cyanoacrylate (SuperBonder®) and Butyl-2-Cyanoacrylate (Histoacryl®) in the process of bone healing in rats: a controlled experimental study

Grant number: 14/08514-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2014
Effective date (End): January 31, 2015
Field of knowledge:Health Sciences - Medicine - Surgery
Principal researcher:Evandro Pereira Palacio
Grantee:Gabriel Guimarães Di Stasi
Home Institution: Faculdade de Medicina de Marília (FAMEMA). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). Marília , SP, Brazil


Background: the use of synthetic adhesives in the medical field has been widespread in recent years. These substances are frequently used in General and Plastic Surgery most of the time to repair lesions in the skin and viscera. On the other hand, there are quite a few data about this subject in the Orthopaedic/Traumatologic field. Thus, the authors propose this research, which objectives are described below. Objectives: to compare, controlled and prospectively, the clinical, biomechanical and histomorphometric results of the use of the Ethyl-2-Cyanoacrylate (SuperBonder®) and the Butyl-2-Cyanoacrylate (Histoacryl®) in the treatment of fractures of the long bones (humerus, femur and tibia) in rats, simulating fractures of tubular bones (phalanges, metacarpals and metatarsals) in humans. Methods: ninety male rats, Wistar lineage, will be used osteotomized (femur, tibia and humerus) bilaterally. The right osteotomized bones will be treated with two drops of SuperBonder® (group A; n=45) or Histoacryl® (group B; n=45), placed directly between the fractured ends. The left osteotomized bones will be treated without any exogenous substance (natural bone healing process), and will serve as controls. Fifteen animals from each group will be euthanized in three moments: 60 days (n=30), 120 days (n=30) and 180 days (n=30). The specimens, after removal of all soft tissues, will be sent for analysis. The femurs will undergo through biomechanical axial compression tests, and the tibias will be sent to three point bending biomechanical tests. The variables: maximum load resistance (N), yield point (N) and stiffness coefficient (N/mm), will be studied in both cases. The humerus will be sent to histomorphometric analysis, which will analyze: the total amount of osteoclasts (n-1), the total amount of osteoblasts (n-1), total amount of abnormal cells (n-1) and the total area of callus (¼m2). The clinical results analysis will be performed by the biweekly body mass measurement (g), throughout the experiment. Results: the results will provide new perspectives for research and treatment of fractures of the hand/feet bones in humans, decreasing the financial costs, the sequelae rates and the hospital stay length of the patients. (AU)