Polymer-Peptide Conjugates for hydrogel formulation and its usage in detection

• Grant number: 15/24018-1
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2016
• End date: March 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Wendel Andrade Alves
• Grantee: Wendel Andrade Alves
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Santo André , SP, Brazil
• City of the host institution: Santo André
• Associated researchers: Emerson Rodrigo da Silva ; Eudes Eterno Fileti ; Sivoney Ferreira de Souza
• Associated research grant(s): 16/50188-4 - Fabrication and characterization of peptide-polymer hybrid hydrogels for bioanalytical applications, AP.R SPRINT

Abstract

In the current project, we intent to investigate the immobilization of Leishmania antigens in hydrogels based on L-phenylalanine (F) and L-arginine (R), covalently bound to synthetic polymer polyethylene glycol (PEG) and poloxamers, aiming developing immunosensors. Specifically, our goal is the search of information on the nucleation, variation of viscoelasticity and stabilization of Leishmania antigens during the process of the peptide hydrogelation contains PEG polymer, by using a wide set of experimental techniques encompassing several spectroscopies, multilevel microscopy and light and X-rays scattering. We will determine few key-variables such as critical aggregation concentrations, and kinetic constants. Physicochemical parameters of the medium wherein the self-assembly occurs will be modulated by varying the ionic strength, pH and polarity of the solvent. Through this procedure, we intend to establish a panorama of the interplay of inter- and intra-molecular forces and thus contributing for understanding these phenomena of great relevance in systems of biological interest, and future application in diagnoses. (AU)

Scientific publications

(22)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FREITAS, R. S.; ALVES, W. A.; PADUAN-FILHO, A.. Magnetic-field-induced ordered phase in the chloro-bridged copper(II) dimer system [Cu-2(apyhist)(2)Cl-2](ClO4)(2). Physical Review B, v. 95, n. 18, MAY 22 2017. (15/24018-1, 15/16191-5)

SOUZA, SIVONEY F.; KOGIKOSKI, JR., SERGIO; SILVA, EMERSON R.; ALVES, WENDEL A.. Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection. Journal of the Brazilian Chemical Society, v. 28, n. 9, p. 1619-1629, SEP 2017. (08/57805-2, 12/01933-8, 13/12674-6, 15/24018-1)

KOGIKOSKI, JR., SERGIO; KHANRA, SOMA; ALVES, WENDEL A.; GUHA, SUCHISMITA. SERS active self-assembled diphenylalanine micro/nanostructures: A combined experimental and theoretical investigation. Journal of Chemical Physics, v. 147, n. 8, AUG 28 2017. (16/14507-8, 12/01933-8, 15/24018-1)

ROJAS, JOSE EDUARDO U.; GERBELLI, BARBARA B.; RIBEIRO, ANDERSON O.; NANTES-CARDOSO, ISELI L.; GIUNTINI, FRANCESCA; ALVES, WENDEL A.. Silk fibroin hydrogels for potential applications in photodynamic therapy. Biopolymers, v. 110, n. 2, FEB 2019. (17/02317-2, 15/24018-1, 14/18527-8, 14/50972-1)

SILVA, EMERSON R.; LISTIK, EDUARDO; HAN, SANG W.; ALVES, WENDEL A.; SOARES, BRUNA M.; REZA, MEHEDI; RUOKOLAINEN, JANNE; HAMLEY, IAN W.. Sequence length dependence in arginine/phenylalanine oligopeptides: Implications for self-assembly and cytotoxicity. Biophysical Chemistry, v. 233, p. 1-12, FEB 2018. (16/24409-3, 15/24018-1)

DA COSTA, PAULO VINICIUS F.; DA SILVA, REJANE MARIA P.; SUFFREDINI, HUGO B.; ALVES, WENDEL A.. Poly-L-Arginine-Modified Boron-Doped Diamond and Glassy Carbon Electrodes for Terbutaline Sulfate Detection. Journal of Nanoscience and Nanotechnology, v. 18, n. 7, p. 4551-4558, JUL 2018. (15/24018-1, 14/50867-3)

MELLO, LUCAS R.; HAMLEY, IAN W.; CASTELLETTO, VALERIA; GARCIA, BIANCA B. M.; LOURENCO, THIAGO C.; VASSILIADES, SANDRA V.; ALVES, WENDEL A.; HAN, SANG W.; SILVA, EMERSON R.. Self-assembly and intracellular delivery of DNA by a truncated fragment derived from the Trojan peptide Penetratin. SOFT MATTER, v. 16, n. 20, p. 4746-4755, MAY 28 2020. (14/50867-3, 17/02317-2, 16/24409-3, 15/24018-1)

FREITAS, R. S.; ALVES, W. A.; PADUAN-FILHO, A.. Magnetic-field-induced ordered phase in the chloro-bridged copper(II) dimer system [Cu-2(apyhist)(2)Cl-2](ClO4)(2). PHYSICAL REVIEW B, v. 95, n. 18, p. 6-pg., 2017-05-22. (15/24018-1, 15/16191-5)

SOUZA, SIVONEY F.; KOGIKOSKI, SERGIO, JR.; SILVA, EMERSON R.; ALVES, WENDEL A.. Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection. Journal of the Brazilian Chemical Society, v. 28, n. 9, p. 11-pg., 2017-09-01. (13/12674-6, 08/57805-2, 15/24018-1, 12/01933-8)

SOUZA, JULIANA S.; PINHEIRO, MAURICIO V. B.; KRAMBROCK, KLAUS; ALVES, WENDEL A.. Dye Degradation Mechanisms Using Nitrogen Doped and Copper(II) Phthalocyanine Tetracarboxylate Sensitized Titanate and TiO2 Nanotubes. Journal of Physical Chemistry C, v. 120, n. 21, p. 11561-11571, JUN 2 2016. (15/24018-1, 11/17938-6, 13/12997-0, 08/57805-2)

KARPEL, RICHARD L.; LIBERATO, MICHELLE DA SILVA; CAMPEIRO, JOANA D.; BERGEON, LORNA; SZYCHOWSKI, BRIAN; BUTLER, ANDREW; MARINO, GIOVANNI; CUSIC, JOELLE F.; GONCALVES DE OLIVEIRA, LILIAN CAROLINE; OLIVEIRA, EDUARDO B.; et al. Design and characterization of crotamine-functionalized gold nanoparticles. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 163, p. 1-8, MAR 1 2018. (15/24018-1, 13/13392-4, 17/02413-1)

PELIN, JULIANE N. B. D.; GATTO, EMANUELA; VENANZI, MARIANO; CAVALIERI, FRANCESCA; OLIVEIRA, CRISTIANO L. P.; MARTINHO, HERCULANO; SILVA, EMERSON R.; AGUILAR, ANDREA M.; SOUZA, JULIANA S.; ALVES, WENDEL A.. Hybrid Conjugates Formed between Gold Nanoparticles and an Amyloidogenic Diphenylalanine-Cysteine Peptide. CHEMISTRYSELECT, v. 3, n. 24, p. 6756-6765, JUN 29 2018. (15/24018-1, 15/20446-9, 17/02317-2, 16/24409-3, 14/50867-3)

GERBELLI, BARBARA BIANCA; DA SILVA, EMERSON RODRIGO; SOARES, BRUNA MIRANDA; ALVES, WENDEL ANDRADE; DE OLIVEIRA, ELISABETH ANDREOLI. Multilamellar-to-Unilamellar Transition Induced by Diphenylalanine in Lipid Vesicles. Langmuir, v. 34, n. 5, p. 2171-2179, FEB 6 2018. (15/24018-1, 16/24409-3)

SILVA, EMERSON R.; COONEY, GARY; HAMLEY, IAN W.; ALVES, WENDEL A.; LEE, SHANNON; O'CONNOR, BRENDAN F.; REZA, MEHEDI; RUOKOLAINEN, JANNE; WALLS, DERMOT. Structural behaviour and gene delivery in complexes formed between DNA and arginine-containing peptide amphiphiles. SOFT MATTER, v. 12, n. 45, p. 9158-9169, 2016. (15/24018-1, 13/12674-6, 14/03514-8)

SOARES, B. M.; AGUILAR, A. M.; SILVA, E. R.; COUTINHO-NETO, M. D.; HAMLEY, I. W.; REZA, M.; RUOKOLAINEN, J.; ALVES, W. A.. Chiral organocatalysts based on lipopeptide micelles for aldol reactions in water. Physical Chemistry Chemical Physics, v. 19, n. 2, p. 1181-1189, 2017. (15/24018-1, 08/57805-2, 13/12674-6)

CASTELLETTO, VALERIA; EDWARDS-GAYLE, CHARLOTTE J. C.; HAMLEY, IAN W.; PELIN, JULIANE N. B. D.; ALVES, WENDEL A.; AGUILAR, ANDREA M.; SEITSONEN, JANI; RUOKOLAINEN, JANNE. Self-Assembly of a Catalytically Active Lipopeptide and Its Incorporation into Cubosomes. ACS APPLIED BIO MATERIALS, v. 2, n. 8, p. 9-pg., 2019-08-19. (15/24018-1, 17/02317-2, 15/20446-9, 14/50867-3)

KOGIKOSKI, JR., SERGIO; LIBERATO, MICHELLE S.; FACTORI, IRINA M.; DA SILVA, EMERSON R.; OLIVEIRA, CRISTIANO L. P.; ANDO, ROMULO A.; ALVES, WENDEL A.. Polycaprolactone-Polyaniline Blend: Effects of the Addition of Cysteine on the Structural and Molecular Properties. Journal of Physical Chemistry C, v. 121, n. 1, p. 863-877, JAN 12 2017. (08/57805-2, 13/12997-0, 12/01933-8, 12/15481-1, 15/24018-1, 13/12674-6)

HAMLEY, IAN W.; BURHOLT, SAMUEL; HUTCHINSON, JESSICA; CASTELLETTO, VALERIA; DA SILVA, EMERSON RODRIGO; ALVES, WENDEL; GUTFREUND, PHILIPP; PORCAR, LIONEL; DATTANI, RAJEEV; HERMIDA-MERINO, DANIEL; et al. Shear Alignment of Bola-Amphiphilic Arginine-Coated Peptide Nanotubes. Biomacromolecules, v. 18, n. 1, p. 141-149, JAN 2017. (13/12674-6, 15/24018-1)

SOUZA, MARCIA I.; PRIETO, TATIANA; RODRIGUES, TIAGO; FERREIRA, FABIO F.; NASCIMENTO, FRANCISCO B.; RIBEIRO, ANDERSON O.; SILVA, EMERSON R.; GIUNTINI, FRANCESCA; ALVES, WENDEL A.. Conjugation with L, L-diphenylalanine Self-Assemblies Enhances In Vitro Antitumor Activity of Phthalocyanine Photosensitizer. SCIENTIFIC REPORTS, v. 7, OCT 13 2017. (15/24018-1, 14/50867-3, 14/50972-1, 16/24409-3)

MELLO, LUCAS R.; HAMLEY, IAN W.; MIRANDA, ANTONIO; ALVES, WENDEL A.; SILVA, EMERSON R.. beta-sheet assembly in amyloidogenic glutamic acid nanostructures: Insights from X-ray scattering and infrared nanospectroscopy. JOURNAL OF PEPTIDE SCIENCE, v. 25, n. 6, JUN 2019. (15/24018-1, 14/50867-3, 17/02317-2, 16/24409-3)

MELLO, LUCAS R.; AGUIAR, RODRIGO B.; YAMADA, RENATA Y.; MORAES, JANE Z.; HAMLEY, IAN W.; ALVES, WENDEL A.; REZA, MEHEDI; RUOKOLAINEN, JANNE; SILVA, EMERSON R.. Amphipathic design dictates self-assembly, cytotoxicity and cell uptake of arginine-rich surfactant-like peptides. JOURNAL OF MATERIALS CHEMISTRY B, v. 8, n. 12, p. 2495-2507, MAR 28 2020. (15/24018-1, 14/50867-3, 17/02317-2, 16/24409-3)

SOUZA, JULIANA S.; ALVES, WENDEL A.. Influence of Preparation Methodology on the Photocatalytic Activity of Nitrogen Doped Titanate and TiO2 Nanotubes. Journal of Nanoscience and Nanotechnology, v. 20, n. 9, p. 5390-5401, SEP 2020. (17/11395-7, 14/50867-3, 15/24018-1)