Biosensors based on amphiphilic peptides for detection and diagnosis of diseases

• Grant number: 18/05888-3
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): June 01, 2018
• Effective date (End): January 31, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Wendel Andrade Alves
• Grantee: Barbara Bianca Gerbelli
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated research grant: 17/02317-2 - Interfaces in materials: electronic, magnetic, structural and transport properties, AP.TEM
• Associated scholarship(s): 20/02192-8 - Structural investigation of lipid and amphiphilic peptides via Langmuir and Langmuir-Blodgett films, BE.EP.PD

Abstract

The main goal of the project is to develop a portable high sensitivity semiconductor transistor device for the diagnosis of human visceral leishmaniasis, a highly fatal parasitic infection, affecting mainly tropical countries. Early diagnosis is essential to prevent patient death. The chip will be based on the immobilization of synthetic peptides made from epitopes of Leishmania infantum, covalently bound into polymer gels. This will be the core of the sensitivity detection. Several complementary approaches will be used to characterize the transistors, mainly x-ray and electron microscopy, both performed in national facilities. Finally, the chips will be tested and validated through comparison with well-established diagnostic procedures (which are however time-consuming and expensive for broad use). The development of the diagnosis will involve complementary expertise from the different team members.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GERBELLI, BARBARA B.; OLIVEIRA, CRISTIANO L. P.; SILVA, EMERSON R.; HAMLEY, IAN W.; ALVES, WENDEL A.. Amyloid Formation by Short Peptides in the Presence of Dipalmitoylphosphatidylcholine Membranes. Langmuir, v. 36, n. 48, p. 14793-14801, DEC 8 2020. (17/02317-2, 19/20907-7, 18/16092-5, 14/50867-3, 17/27078-0, 18/05888-3)

GERBELLI, BARBARA B.; VASSILIADES, SANDRA V.; ROJAS, JOSE E. U.; PELIN, JULIANE N. B. D.; MANCINI, RODRIGO S. N.; PEREIRA, WALLACE S. G.; AGUILAR, ANDREA M.; VENANZI, MARIANO; CAVALIERI, FRANCESCA; GIUNTINI, FRANCESCA; et al. Hierarchical Self-Assembly of Peptides and its Applications in Bionanotechnology. MACROMOLECULAR CHEMISTRY AND PHYSICS, v. 220, n. 14, SI, JUL 2019. (18/05888-3, 16/50188-4, 17/02317-2, 14/50972-1, 14/50867-3)

PELIN, JULIANE N. B. D.; GERBELLI, BARBARA B.; SOARES, BRUNA M.; AGUILAR, ANDREA M.; ALVES, WENDEL A.. Amyloidogenic model peptides as catalysts for stereoselective aldol reactions. CATALYSIS SCIENCE & TECHNOLOGY, v. 9, n. 16, p. 4304-4313, AUG 21 2019. (14/50867-3, 17/02317-2, 18/05888-3, 15/20446-9)

PELIN, JULIANE N. B. D.; EDWARDS-GAYLE, CHARLOTTE J. C.; MARTINHO, HERCULANO; GERBELLI, BARBARA B.; CASTELLETTO, VALERIA; HAMLEY, IAN W.; ALVES, WENDEL A.. Self-assembled gold nanoparticles and amphiphile peptides: a colorimetric probe for copper(ii) ion detection. DALTON TRANSACTIONS, v. 49, n. 45, p. 16226-16237, DEC 7 2020. (18/05888-3, 15/20446-9, 18/12535-0, 17/02317-2)

MARTINEZ, RENATA MILIANI; OSELIERO FILHO, PEDRO LEONIDAS; GERBELLI, BARBARA BIANCA; MAGALHAES, WAGNER VIDAL; VELASCO, MARIA VALERIA ROBLES; DA SILVA LANNES, SUZANA CAETANO; DE OLIVEIRA, CRISTIANO LUIS PINTO; ROSADO, CATARINA; BABY, ANDRE ROLIM. Influence of the Mixtures of Vegetable Oil and Vitamin E over the Microstructure and Rheology of Organogels. GELS, v. 8, n. 1, JAN 2022. (16/24531-3, 20/02192-8, 19/16169-0, 19/12301-1, 18/05888-3, 20/13204-7)

PELIN, JULIANE N. B. D.; GERBELLI, BARBARA B.; EDWARDS-GAYLE, CHARLOTTE J. C.; AGUILAR, ANDREA M.; CASTELLETTO, VALERIA; HAMLEY, IAN W.; ALVES, WENDEL A.. Amyloid Peptide Mixtures: Self-Assembly, Hydrogelation, Nematic Ordering, and Catalysts in Aldol Reactions. Langmuir, v. 36, n. 11, p. 2767-2774, MAR 24 2020. (14/50867-3, 17/02317-2, 18/05888-3, 18/12535-0, 15/20446-9)

SOARES, BRUNA M.; SODRE, PEDRO T.; AGUILAR, ANDREA M.; GERBELLI, BARBARA B.; PELIN, JULIANE N. B. D.; ARGUELLO, KARINA B.; SILVA, EMERSON R.; DE FARIAS, MARCELO A.; PORTUGAL, RODRIGO V.; SCHMUCK, CARSTEN; et al. Structure optimization of lipopeptide assemblies for aldol reactions in an aqueous medium. Physical Chemistry Chemical Physics, v. 23, n. 18, p. 10953-10963, MAY 14 2021. (17/02317-2, 18/05888-3, 15/20446-9, 14/50867-3)

OSELIERO FILHO, PEDRO LEONIDAS; GERBELLI, BARBARA BIANCA; FORNASIER, FRANCCESCA; CHAVES FILHO, ADRIANO B.; YOSHINAGA, MARCOS YUKIO; MIYAMOTO, SAYURI; MORTARA, LAURA; LACERDA, CAROLINE DUTRA; CUCCOVIA, IOLANDA MIDEA; PIMENTEL, ANDRE SILVA; et al. Structure and Thermotropic Behavior of Bovine- and Porcine-Derived Exogenous Lung Surfactants. Langmuir, v. 36, n. 48, p. 14514-14529, DEC 8 2020. (16/24531-3, 19/12301-1, 18/05888-3, 14/50983-3, 18/16092-5)