The role of gametogenesis on the origin and evolution of new genes

• Grant number: 15/20844-4
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: April 01, 2016
• End date: March 31, 2022
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Maria Dulcetti Vibranovski
• Grantee: Maria Dulcetti Vibranovski
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Antonio Bernardo de Carvalho ; Bernardo Lemos Ferreira da Silva ; Manyuan Long ; Timothy L. Karr ; Yong Edward Zhang
• Associated scholarship(s): 20/05086-4 - Analysis and classification of X-2 chromosomal translocations in Drosophila melanogaster, BP.IC; 19/14878-4 - A technique for constructing complete oligopaints from heterochromatic sequences using Drosophila melanogaster Y chromosome as a model, BP.MS; 19/15212-0 - Study of meiotic X chromosome inactivation in Drosophila spp. spermatogenesis, BP.DD; + associated scholarships 19/10559-1 - Evolution of the meiotic X-chromosome inactivation in Drosophila, BP.MS; 17/26609-2 - Study of meiotic X chromosome inactivation by gene expression analyses of the Drosophila melanogaster spermatogenesis, BP.MS; 17/14923-4 - A study on the functions of new Y-linked genes through expression analysis of Drosophila's spermatogenesis, BP.MS; 16/09378-4 - Evolutionary analysis of new genes in Drosophila through transcriptomes of Gametogenesis, BP.DR - associated scholarships

Abstract

New genes are frequently formed during evolution in a wide variety of organisms playing a key role in the emergence of novel traits. Recently, studies have shown that new genes can quickly assume critical roles in developmental pathways by producing essential structures. Despite their importance, new genes are still seriously under-characterized in functional studies and inconsistently annotated in large-scale genome projects. Hence, biased estimations of gene gain and loss prevent us to establish how mutational processes and selective forces contribute to the source of new genes. Therefore, determining relations between genotype and phenotype in the emergence of a new gene requires current researches to apply a biological developmental system approach and perspective. Gametogenesis is a system of great importance for survival and evolution of species that varies temporally with the development and has a profound impact on new genes. Spermatogenesis, the male gametogenesis, provides half of the raw genetic material for the next generation and is enriched with new genes expression. The main goal of this project is to study processes and evolutionary forces determinant for evolution and origin of new genes. To achieve this goal, I will: 1- investigate the role of mutation and selective processes in the Drosophila and mammal spermatogenesis impacting new gene evolution; 2: take advantage of gametogenesis gene expression to generate unbiased rates of gene origin in Drosophila; 3- investigate new gene origination associated with sex chromosome evolution. I expect that the systems biology approach in an integrated framework will help understand trajectories of new gene origination and its impact in genome evolution, speciation and phenotypic consequences. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAHADEVARAJU, SHARVANI; FEAR, JUSTIN M.; AKEJU, MIRIAM; GALLETTA, BRIAN J.; PINHEIRO, MARA M. L. S.; AVELINO, CAMILA C.; CABRAL-DE-MELLO, DIOGO C.; CONLON, KATIE; DELL'ORSO, STAFANIA; DEMERE, ZELALEM; et al. Dynamic sex chromosome expression in Drosophila male germ cells. NATURE COMMUNICATIONS, v. 12, n. 1, FEB 9 2021. (15/20844-4, 17/14923-4, 17/26609-2, 15/16661-1, 13/08028-1, 19/15212-0)

MOREIRA DE MELLO, JOANA C.; FERNANDES, GUSTAVO R.; VIBRANOVSKI, MARIA D.; PEREIRA, LYGIA V.. Early X chromosome inactivation during human preimplantation development revealed by single-cell RNA-sequencing. SCIENTIFIC REPORTS, v. 7, SEP 7 2017. (15/20844-4, 09/17481-6, 13/08135-2, 15/03610-0)

RAICES, JULIA B.; OTTO, PAULO A.; VIBRANOVSKI, MARIA D.. Haploid selection drives new gene male germline expression. Genome Research, v. 29, n. 7, p. 1115-1122, JUL 2019. (15/20844-4, 13/08028-1, 13/06117-7)

RODRIGUES, MURILLO F.; VIBRANOVSKI, MARIA D.; COGNI, RODRIGO. Clinal and seasonal changes are correlated in Drosophila melanogaster natural populations. Evolution, v. 75, n. 8, JUL 2021. (17/02206-6, 17/06374-0, 15/20844-4, 13/25991-0, 16/01354-9)

FRANCA, GUSTAVO S.; HINSKE, LUDWIG C.; GALANTE, PEDRO A. F.; VIBRANOVSKI, MARIA D.. Unveiling the Impact of the Genomic Architecture on the Evolution of Vertebrate microRNAs. FRONTIERS IN GENETICS, v. 8, MAR 21 2017. (15/20844-4)