Molecular phylogeny of Calyptratae Diptera using traditional and next generation sequencing.

• Grant number: 15/10788-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2016
• End date: October 31, 2018
• Field of knowledge: Biological Sciences - Zoology - Taxonomy of Recent Groups
• Principal Investigator: Silvio Shigueo Nihei
• Grantee: Silvio Shigueo Nihei
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Ana Maria Lima de Azeredo-Espin ; Cátia Antunes de Mello Patiu ; Chuntian Zhang ; Claudio Jose Barros de Carvalho ; Diana Lucia Grisales Ochoa ; Dong Zhang ; Fernando Portella de Luna Marques ; Gustavo Graciolli ; Kirstern Lica Follmann Haseyama ; Márcia Souto Couri ; Marco Antonio Tonus Marinho ; Maria Cristina Esposito ; Martha Wolff ; Tatiana Teixeira Torres

Abstract

The clade Calyptrata in Diptera comprises 15 families and over 20,000 species, about 12% of the world Diptera diversity. The Calyptratae are divided into three superfamilies: Hippoboscoidea, Muscoidea and Oestroidea. The Neotropical diversity of the group comprises 5,000 species, 1/4 of world diversity. Although the monophyly is consistently corroborated, there are several problems to be cleared in Calyptrata, such as the superfamilies relationships, the monophyly of superfamilies, the families relationships, and monophyly of families, and these questions are very controversial within Diptera higher-level phylogeny. Moreover, there is a recurrent problem in the higher-level molecular phylogenies of Diptera ¯ for family level and all levels above ¯ that is the downsampling and misrepresentation of the Neotropical fauna. Articles published by authors from Northern Hemisphere include an average Neotropical sampling of 9%, while the authors from Southern Hemisphere include 48% (average) of Neotropical taxa in their phylogenies. The aim of this project is to perform a molecular phylogeny of Diptera Calyptrata based on molecular data obtained through PCR-amplified molecular markers (traditional or Sanger sequencing) and through large-scale sequencing (Next Generation Sequencing - NGS). In this project, there will be two innovative and impactful factors for the phylogenetic knowledge of Diptera and of Calyptrata, the first is the increment of Neotropical sampling (no phylogeny of Calyptrata have ever included a meaningful Neotropical sampling), and the second is the pioneering the large-scale sequencing (there is no phylogenetic study of Diptera using NGS published so far). This project involves a network composed of 15 researchers, 2 post-docs, 4 PhD students and 2 technicians, gathering 14 different Brazilian (9) and foreign (5) institutions. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARBONARO, FABIO AUGUSTO; LANGER, MAX CARDOSO; NIHEI, SILVIO SHIGUEO; FERREIRA, GABRIEL DE SOUZA; GHILARDI, RENATO PIRANI. Inferring ancestral range reconstruction based on trilobite records: a study-case on Metacryphaeus (Phacopida, Calmoniidae). SCIENTIFIC REPORTS, v. 8, OCT 12 2018. (13/09683-3, 15/10788-0, 16/03934-2, 14/03825-3, 16/18275-4, 14/25379-5)

MARCELO DOMINGOS DE SANTIS; SILVIO SHIGUEO NIHEI. The first Dexiinae from Mauritius: the description of a new species of Pandelleia Villeneuve, 1907 (Diptera, Tachinidae). Papéis Avulsos de Zoologia (São Paulo), v. 61, 2021-12-08. (15/10788-0)

DEIVYS MOISES ALVAREZ-GARCIA; MARÍA PAULA SIERRA-JUEZ; DIMAS KENJI SAKAMOTO; SILVIO SHIGUEO NIHEI. Ultrastructural analysis of the antennae of Hemilucilia segmentaria (Diptera: Calliphoridae), a blowfly of forensic importance. Papéis Avulsos de Zoologia (São Paulo), v. 62, 2022-11-07. (15/10788-0)

NIHEI, SILVIO S.; LIU, GENTING; WANG, QIKE; LIU, XIANUI; LI, XINYU; PANG, XIUNAN; ALVAREZ-GARCIA, DEIVYS; ZHANG, DONG. Comparative morphology of antennal ultrastructure in Tachinidae parasitoid flies (Diptera): The phylogenetic importance of antennal sensilla. ARTHROPOD STRUCTURE & DEVELOPMENT, v. 71, p. 15-pg., 2022-08-26. (15/10788-0)

KROLOW, T. K.; HENRIQUES, A. L.; GONZALEZ, C. R.; NIHEI, S. S.. Comments on the Classification of Neotropical Scionini (Tabanidae) with a Description of a New Genus. Neotropical Entomology, v. 49, n. 3, p. 8-pg., 2020-05-12. (15/10788-0)

WOOD, CAMILA T.; NIHEI, SILVIO S.; ARAUJO, PAULA B.. Woodlice and their parasitoid flies: revision of Isopoda (Crustacea, Oniscidea) - Rhinophoridae (Insecta, Diptera) interaction and first record of a parasitized Neotropical woodlouse species. ZOOKEYS, n. 801, p. 401-414, DEC 3 2018. (15/10788-0)

GUDIN, FILIPE M.; NIHEI, SILVIO S.. Taxonomic revision of the Neotropical genus Ormiophasia Townsend, 1919 (Diptera: Tachinidae), with the description of eight new species. Zootaxa, v. 4643, n. 1, p. 1-74, JUL 22 2019. (15/10788-0, 14/01952-8, 12/17847-3)

DE CAMPOS, LUCAS DENADAI; SOUZA-DIAS, PEDRO G. B.; NIHEI, SILVIO S.. Taxonomic review of Eidmanacris Chopard, 1956 (Orthoptera: Grylloidea: Phalangopsidae). Zootaxa, v. 4321, n. 1, p. 1-93, SEP 19 2017. (14/09863-4, 15/19112-9, 15/10788-0)

DE CAMPOS, LUCAS DENADAI; SOUZA-DIAS, PEDRO GUILHERME BARRIOS DE; DESUTTER-GRANDCOLAS, LAURE; SHIGUEO NIHEI, SILVIO. Colonization of different biomes drove the diversification of the Neotropical Eidmanacris crickets (Insecta: Orthoptera: Grylloidea: Phalangopsidae). PLoS One, v. 16, n. 1, JAN 15 2021. (15/10788-0, 18/23224-5, 10/52314-0, 15/19112-9, 14/09863-4)

WOOD, CAMILA T.; NIHEI, SILVIO S.; ARAUJO, PAULA B.. Woodlice and their parasitoid flies: revision of Isopoda (Crustacea, Oniscidea) - Rhinophoridae (Insecta, Diptera) interaction and first record of a parasitized Neotropical woodlouse species. ZOOKEYS, v. N/A, n. 801, p. 14-pg., 2018-12-03. (15/10788-0)

DE SANTIS, MARCELO DOMINGOS; NIHEI, SILVIO SHIGUEO. hylogenetic analysis of the tribe Dufouriini (Diptera: Tachinidae) using a total evidence approach based on adult and immature stage. ARTHROPOD SYSTEMATICS & PHYLOGENY, v. 80, p. 1-38, JAN 21 2022. (10/52314-0, 15/10788-0)

SILVIO S. NIHEI; RODRIGO DE V.P. DIOS. Nomenclatural acts for some Neotropical Tachinidae (Diptera). Papéis Avulsos de Zoologia (São Paulo), v. 56, n. 16, p. 177-181, 2016-00-00. (15/10788-0)

DE SANTIS, MARCELO DOMINGOS; NIHEI, SILVIO SHIGUEO. Two new species of Neosophia Guimaraes, 1982 and first description of female of Neoeuantha sabroskyi Guimaraes, 1982 (Diptera: Tachinidae). Journal of Natural History, v. 53, n. 1-2, p. 109-125, JAN 10 2019. (15/10788-0)

MARCELO D. SANTIS; SILVIO S. NIHEI. Review of the genus Euantha Wulp, 1885 (Diptera: Tachinidae), with key to species and redescription of E. pulchra Wulp, 1891. Anais da Academia Brasileira de Ciências, v. 95, n. 1, 2023-05-19. (15/10788-0)

PEREIRA, THALLES PLATINY LAVINSCKY; BROWN, BRIAN, V; NIHEI, SILVIO S.. The Neotropical ant-parasitic genus Neodohrniphora Malloch (Diptera: Phoridae): a taxonomic revision with seven new species and key to species. Insect Systematics and Evolution, v. 53, n. 3, p. 31-pg., 2022-05-01. (10/52314-0, 15/10788-0)