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Molecular phylogeny of Calyptratae Diptera using traditional and next generation sequencing

Grant number: 15/10788-0
Support type:Regular Research Grants
Duration: June 01, 2016 - October 31, 2018
Field of knowledge:Biological Sciences - Zoology - Taxonomy of Recent Groups
Principal Investigator:Silvio Shigueo Nihei
Grantee:Silvio Shigueo Nihei
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Ana Maria Lima de Azeredo-Espin ; Cátia Antunes de Mello Patiu ; Chuntian Zhang ; Claudio Jose Barros de Carvalho ; Diana Lucia Grisales Ochoa ; Dong Zhang ; Fernando Portella de Luna Marques ; Gustavo Graciolli ; Kirstern Lica Follmann Haseyama ; Márcia Souto Couri ; Marco Antonio Tonus Marinho ; Maria Cristina Esposito ; Martha Wolff ; Tatiana Teixeira Torres


The clade Calyptrata in Diptera comprises 15 families and over 20,000 species, about 12% of the world Diptera diversity. The Calyptratae are divided into three superfamilies: Hippoboscoidea, Muscoidea and Oestroidea. The Neotropical diversity of the group comprises 5,000 species, 1/4 of world diversity. Although the monophyly is consistently corroborated, there are several problems to be cleared in Calyptrata, such as the superfamilies relationships, the monophyly of superfamilies, the families relationships, and monophyly of families, and these questions are very controversial within Diptera higher-level phylogeny. Moreover, there is a recurrent problem in the higher-level molecular phylogenies of Diptera ¯ for family level and all levels above ¯ that is the downsampling and misrepresentation of the Neotropical fauna. Articles published by authors from Northern Hemisphere include an average Neotropical sampling of 9%, while the authors from Southern Hemisphere include 48% (average) of Neotropical taxa in their phylogenies. The aim of this project is to perform a molecular phylogeny of Diptera Calyptrata based on molecular data obtained through PCR-amplified molecular markers (traditional or Sanger sequencing) and through large-scale sequencing (Next Generation Sequencing - NGS). In this project, there will be two innovative and impactful factors for the phylogenetic knowledge of Diptera and of Calyptrata, the first is the increment of Neotropical sampling (no phylogeny of Calyptrata have ever included a meaningful Neotropical sampling), and the second is the pioneering the large-scale sequencing (there is no phylogenetic study of Diptera using NGS published so far). This project involves a network composed of 15 researchers, 2 post-docs, 4 PhD students and 2 technicians, gathering 14 different Brazilian (9) and foreign (5) institutions. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUDIN, FILIPE M.; NIHEI, SILVIO S. Taxonomic revision of the Neotropical genus Ormiophasia Townsend, 1919 (Diptera: Tachinidae), with the description of eight new species. Zootaxa, v. 4643, n. 1, p. 1-74, JUL 22 2019. Web of Science Citations: 0.
DE SANTIS, MARCELO DOMINGOS; NIHEI, SILVIO SHIGUEO. Two new species of Neosophia Guimaraes, 1982 and first description of female of Neoeuantha sabroskyi Guimaraes, 1982 (Diptera: Tachinidae). Journal of Natural History, v. 53, n. 1-2, p. 109-125, JAN 10 2019. Web of Science Citations: 0.
CARBONARO, FABIO AUGUSTO; LANGER, MAX CARDOSO; NIHEI, SILVIO SHIGUEO; FERREIRA, GABRIEL DE SOUZA; GHILARDI, RENATO PIRANI. Inferring ancestral range reconstruction based on trilobite records: a study-case on Metacryphaeus (Phacopida, Calmoniidae). SCIENTIFIC REPORTS, v. 8, OCT 12 2018. Web of Science Citations: 1.
DE CAMPOS, LUCAS DENADAI; SOUZA-DIAS, PEDRO G. B.; NIHEI, SILVIO S. Taxonomic review of Eidmanacris Chopard, 1956 (Orthoptera: Grylloidea: Phalangopsidae). Zootaxa, v. 4321, n. 1, p. 1-93, SEP 19 2017. Web of Science Citations: 1.
SILVIO S. NIHEI; RODRIGO DE V.P. DIOS. Nomenclatural acts for some Neotropical Tachinidae (Diptera). Papéis Avulsos de Zoologia (São Paulo), v. 56, n. 16, p. -, 2016.

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