Laccase application in the modification of biomaterials and tissue for ophthalmic use and evaluation of the production of it by endophytic fungi of Manihot Esculenta, Crantz

• Grant number: 15/19273-2
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2016
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Andreia de Araújo Morandim
• Grantee: Andreia de Araújo Morandim
• Host Institution: Centro Universitário FEI (UNIFEI). Campus de São Bernardo do Campo. São Bernardo do Campo , SP, Brazil
• Associated researchers: Octaviano Magalhães Junior ; Patrícia Alessandra Bersanetti ; Paulo Schor

Abstract

Endophytic fungi have shown promising sources for obtaining enzymes with biotechnological potential. Thus, during the course of this project will be obtained and purified strains of endophytic fungi associated with cassava leaves, Manihot esculenta Crantz. After obtaining the lines, it will be cultured in potato dextrose agar (PDA). The pure strains will be cultured using a specific medium for the production of laccase. For this, it will be used a culture medium composed of malt extract, Tween 80, copper sulfate and 2,5-xylidine. After growth, fungi will be separated and subjected to enzymatic extraction process to obtaining laccases. During this stage, the best extraction buffer is checked and also the presence of the enzyme in each previously isolated and cultured lineage. In parallel, studies related to formation of crosslinked in corneas and in hydrogels for the replacement of vitreous will be performed using laccase. Thus, the influence of the same the formation of cross-links will be verified, and determined of the optimal pH, reaction time, the enzyme concentration and temperature. For this, it being used a statistical planning. Physical, chemical, mechanical, thermal and surface tests will be conducted to evaluate the presence of cross-link in corneas and in hydrogels. Finally, the extracts that show the presence of laccases will be tested under conditions determined beforehand by performing a pre-purification of the enzyme of interest. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERSANETTI, PATRICIA ALESSANDRA; ESCOBAR, VICTOR HUGO; NOGUEIRA, REGINA FREITAS; ORTEGA, FERNANDO DOS SANTOS; SCHOR, PAULO; MORANDIM-GIANNETTI, ANDREIA DE ARAUJO. Enzymatically obtaining hydrogels of PVA crosslinked with ferulic acid in the presence of laccase for biomedical applications. EUROPEAN POLYMER JOURNAL, v. 112, p. 610-618, MAR 2019. (15/19273-2)

GARCIA, VITOR STABILE; BERSANETTI, PATRICIA ALESSANDRA; MORANDIM-GIANNETTI, ANDREIA DE ARAUJO. Peptidases production by fungi obtained from Manihot esculenta Crantz waste and its application in gluten hydrolysis. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 37, OCT 2021. (15/19273-2)

MORANDIM-GIANNETTI, ANDREIA DE ARAUJO; WECCHI, PATRICK DE OLIVEIRA; SILVERIO, PAULA DE ARAUJO; CARLSTRON, REGINA; BERSANETTI, PATRICIA ALESSANDRA. Attainment and characterization of carboxymethyl chitosan hydrogels by enzymatic cross-linking. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, v. 138, n. 5, p. 3635-3643, DEC 2019. (15/19273-2)

MORANDIM-GIANNETTI, ANDREIA DE ARAUJO; CARVALHO, TALEESA SOUZA; DE ANDRADE NETO, JOSE CARLOS; BERSANETTI, PATRICIA ALESSANDRA. DSC evaluation of cross-link development induced by laccases in corneas. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, v. 148, n. 1, p. 6-pg., 2022-11-25. (15/19273-2)