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Bull semen and embryos microRNAs profile and these correlation with fertility

Abstract

The bull semen has several molecules not yet described. Among them are the microRNAs, recognized as fundamental for fertilization and early embryonic development. However, there are few studies evaluating the profile of seminal miRNAs and fertility in cattle. The study hypothesis is that miRNAs present in semen are associated with fertility. For this, the study aims to investigate the miRNAs present in sperm and relate them to the embryonic development in vitro and in vivo, and fertility rates in the field. Three experiments will be performed. The first experiment will assess sperm quality and sperm miRNAs profile of commercial doses of semen. For this, it will be used three semen batches of six bulls (n=18) with different fertility rates previously confirmed by field studies. Each replicate will be evaluated to characteristics of sperm kinetics, plasma and acrosome membranes integrity and potential of mitochondrial membrane and also to miRNAs sperm profile. In the second experiment, the same batches will be used for in vitro and in vivo embryos production. The data (blastocyst rate and miRNAs profile of embryos) will be compared between the high and low fertility groups established in the experiment 1. Embryos in vitro and in vivo will be also compared to miRNAs profile. In the third experiment, it will be frozen three ejaculates from six bulls (n=18). Fertility rates from these bulls will be unknown. It will be investigate sperm miRNAs profile and blastocyst rate. Data from different experiments will be submitted to ANOVA with 5% of significance level. It is expected to find miRNAs associated with fertility. This project results will be used to create a new technique to evaluate bull semen. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES ALVES, MAIRA BIANCHI; DE ARRUDA, RUBENS PAES; DE BEM, TIAGO HENRIQUE CAMARA; FLOREZ-RODRIGUEZ, SHIRLEY ANDREA; DE SA FILHO, MANOEL FRANCISCO; BELLEANNEE, CLEMENCE; MEIRELLES, FLAVIO VIEIRA; DA SILVEIRA, JULIANO COELHO; PERECIN, FELIPE; CARVALHO CELEGHINI, ENEIVA CARLA. Sperm-borne miR-216b modulates cell proliferation during early embryo development via K-RAS. SCIENTIFIC REPORTS, v. 9, . (16/05395-1, 15/09154-6, 16/23243-4)
GARCIA-OLIVEROS, LAURA NATALY; DE ARRUDA, RUBENS PAES; BATISSACO, LEONARDO; GUILGER GONZAGA, VITOR HUGO; MOREIRA NOGUEIRA, VINICIUS JOSE; FLOREZ-RODRIGUEZ, SHIRLEY ANDREA; ALMEIDA, FLAVIA DOS SANTOS; RODRIGUES ALVES, MAIRA BIANCHI; COSTA PINTO, SAMARA CRISTINE; NICHI, MARCILIO; et al. Heat stress effects on bovine sperm cells: a chronological approach to early findings. INTERNATIONAL JOURNAL OF BIOMETEOROLOGY, v. 64, n. 8, . (14/12757-1, 16/05395-1)
CARVALHO CELEGHINI, ENEIVA CARLA; RODRIGUES ALVES, MAIRA BIANCHI; DE ARRUDA, RUBENS PAES; DE REZENDE, GABRIELA MARQUES; FLOREZ-RODRIGUEZ, SHIRLEY ANDREA; DE SA FILHO, MANOEL FRANCISCO. Efficiency of CellROX deep red (R) and CellROX orange (R) fluorescent probes in identifying reactive oxygen species in sperm samples from high and low fertility bulls. ANIMAL BIOTECHNOLOGY, v. 32, n. 1, . (16/05395-1, 15/09154-6)

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