Genetic, morphological and biological diversity, phylogenetic relationships and ta...
| Grant number: | 15/25592-3 |
| Support type: | Regular Research Grants |
| Duration: | August 01, 2016 - July 31, 2018 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine |
| Principal Investigator: | Arlei Marcili |
| Grantee: | Arlei Marcili |
| Home Institution: | Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Assoc. researchers: | Antonio Humberto Hamad Minervino ; Marcia Aparecida Speranca ; Pablo Henrique Nunes ; Richard de Campos Pacheco ; Solange Maria Gennari |
Abstract
Trypanosoma genus belongs to the Trypanosomatidae Family, comprising flagellate protozoa of Kinetoplastida order. The trypanosomes parasitize a wide range of hosts, including almost all orders of invertebrates and vertebrates. The Trypanosoma species are parasites of all vertebrate (fish, amphibians, reptiles, birds and mammals) with life cycles alternating between vertebrates and invertebrates hosts. Most species develops in blood-sucking arthropods. The species of the Trypanosoma genus, parasites of mammals, were separated into sections Salivaria and Stercoraria, according to the development in the invertebrate host and consequently, to the route of infective forms elimination by the vector. Stercoraria section includes Schizotrypanum subgenus (species type T. cruzi), Herpetosoma (T. lewisi) and Megatrypanum (T. theileri). Only Schizotrypanum proved to be a monophyletic group. The Herpetosoma and Megatrypanum subgenres have proven polyphyletic. In recent years several species of trypanosomes isolated from wild animals in Australia, Africa, China and Brazil have been reported and included in phylogenetic studies, but more studies are necessary considering the wide range of vertebrate and invertebrate hosts and the broad distribution. Thus, this project has as main objective the knowledge and description of parasites from Trypanosoma genus diversity in several classes of wild animals captured in different biomes. New and non-characterized Trypanosoma isolates deposited at the trypanosomatide Collection will be characterized by light microscopy, scanning and transmission electron microscopy, biological behavior in different cell lines and phylogenetic studies based on SSUrDNA and gGAPDH genes for positioning of new species and the Cytochrome B and ITS1 genes for analysis of intraspecific variability. (AU)