Taxonomy and phylogeny of Trypanosoma species: description of a new species from wild animals

• Grant number: 15/25592-3
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2016
• End date: July 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Arlei Marcili
• Grantee: Arlei Marcili
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Antonio Humberto Hamad Minervino ; Marcia Aparecida Speranca ; Pablo Henrique Nunes ; RICHARD DE CAMPOS PACHECO ; Solange Maria Gennari

Abstract

Trypanosoma genus belongs to the Trypanosomatidae Family, comprising flagellate protozoa of Kinetoplastida order. The trypanosomes parasitize a wide range of hosts, including almost all orders of invertebrates and vertebrates. The Trypanosoma species are parasites of all vertebrate (fish, amphibians, reptiles, birds and mammals) with life cycles alternating between vertebrates and invertebrates hosts. Most species develops in blood-sucking arthropods. The species of the Trypanosoma genus, parasites of mammals, were separated into sections Salivaria and Stercoraria, according to the development in the invertebrate host and consequently, to the route of infective forms elimination by the vector. Stercoraria section includes Schizotrypanum subgenus (species type T. cruzi), Herpetosoma (T. lewisi) and Megatrypanum (T. theileri). Only Schizotrypanum proved to be a monophyletic group. The Herpetosoma and Megatrypanum subgenres have proven polyphyletic. In recent years several species of trypanosomes isolated from wild animals in Australia, Africa, China and Brazil have been reported and included in phylogenetic studies, but more studies are necessary considering the wide range of vertebrate and invertebrate hosts and the broad distribution. Thus, this project has as main objective the knowledge and description of parasites from Trypanosoma genus diversity in several classes of wild animals captured in different biomes. New and non-characterized Trypanosoma isolates deposited at the trypanosomatide Collection will be characterized by light microscopy, scanning and transmission electron microscopy, biological behavior in different cell lines and phylogenetic studies based on SSUrDNA and gGAPDH genes for positioning of new species and the Cytochrome B and ITS1 genes for analysis of intraspecific variability. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, JULIANA I. G. S.; DA COSTA, ANDREA P.; NUNES, PABLO HENRIQUE; RAMIREZ, DIEGO; FOURNIER, GISLENE F. R.; SARAIVA, DANILO; TONHOSOLO, RENATA; MARCILI, ARLEI. New Trypanosoma species, Trypanosoma gennarii sp nov., from South American marsupial in Brazilian Cerrado. Acta Tropica, v. 176, p. 249-255, DEC 2017. (15/25592-3)

MARCILI, ARLEI; DA SILVA, RYAN EMILIANO; DA COSTA, VILMA PEREIRA; NIERI-BASTOS, FERNANDA A.; DE FREITAS E AZEVEDO, ROBERTA CARVALHO; MORAES FILHO, JONAS; TONHOSOLO, RENATA. Canine Visceral Leishmaniasis in Sao Paulo, Brazil, the Most Populous City of South America: Isolation, Molecular Diagnosis, and Phylogenetic Inferences. VECTOR-BORNE AND ZOONOTIC DISEASES, v. 20, n. 10, JUN 2020. (15/25592-3)

PEREZ, SERGIO D.; GRUMMER, JARED A.; FERNANDES-SANTOS, RENATA C.; JOSE, CAROLINE TESTA; MEDICI, EMILIA PATRICIA; MARCILI, ARLEI. Phylogenetics, patterns of genetic variation and population dynamics of Trypanosoma terrestris support both coevolution and ecological host-fitting as processes driving trypanosome evolution. PARASITES & VECTORS, v. 12, n. 1, OCT 11 2019. (15/25592-3)

CAMPOLONGO, CAIO; SILVA, RYAN EMILIANO DA; AZEVEDO, ROBERTA CARVALHO DE FREITAS E; PESENATO, ISABELLA PEREIRA; CARIOCA, ANA CAROLINA FERNANDES; ALVES, BRUNA FARIAS; CASTELLI, GIOVANNA STEFANI NOSBERTO; ONOFRIO, VALERIA CASTILHO; MARCILI, ARLEI. revalence of Leishmania infantum in Dogs from Deforested Areas of the Amazon Biom. VECTOR-BORNE AND ZOONOTIC DISEASES, DEC 2021. (15/25592-3)

ROBERTA CARVALHO DE FREITAS E AZEVEDO; GIOVANNA STEFANI NOSBERTO CASTELLI; RYAN EMILIANO DA SILVA; JACIARA DE OLIVEIRA JORGE COSTA; RENATA TONHOSOLO; EDUARDO ALBERTO REIS; JONAS MORAES FILHO; ARLEI MARCILI. Levantamento de protozoários transmitidos por vetores em cães de um fragmento de Mata Atlântica nos arredores da Barragem Billings, São Paulo, Brasil. Ciência Rural, v. 50, n. 9, 2020-07-10. (15/25592-3)

SOUZA DYONISIO, GUSTAVO HENRIQUE; BATISTA, HELDER RIBEIRO; DA SILVA, RYAN EMILIANO; DE FREITAS E AZEVEDO, ROBERTA CARVALHO; JORGE COSTA, JACIARA DE OLIVEIRA; DE OLIVEIRA MANHAES, INGRIDI BRAZ; TONHOSOLO, RENATA; GENNARI, SOLANGE MARIA; HAMAD MINERVINO, ANTONIO HUMBERTO; MARCILI, ARLEI. Molecular Diagnosis and Prevalence of Trypanosoma vivax (Trypanosomatida: Trypanosomatidae) in Buffaloes and Ectoparasites in the Brazilian Amazon Region. Journal of Medical Entomology, v. 58, n. 1, p. 403-407, JAN 2021. (15/25592-3)