Transcriptome profile analysis during photoperiodic induction in sugarcane by RNA-seq

• Grant number: 16/02679-9
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: February 28, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Luciana Rossini Pinto Machado da Silva
• Grantee: Luciana Rossini Pinto Machado da Silva
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Campinas , SP, Brazil
• City of the host institution: Campinas
• Associated researchers: Elisson Antonio da Costa Romanel ; Maximiliano Salles Scarpari ; Michael dos Santos Brito ; Renato Vicentini dos Santos
• Associated research grant(s): 18/08380-0 - Identification of key target genes for the manipulation of flowering in sugarcane, AP.R SPRINT

Abstract

The Brazilian sugarcane breeding programs has positively impacted the sugarcane industry by the development of productive cultivares adapted to different edaphoclimatic conditions of the country. Flowering is one of the relevant factors for sugarcane. Despite its importance, the knowledge of the genes involved during the photoperiodic induction in sugarcane is still scarce, and the investigation of differentially expressed genes under controlled photoperiodic conditions, in fact, still need to be explored. This project aims to study the transcriptome of a sugarcane cultivar during flowering induction via RNA-seq (RNA-sequencing) technique to identify differentially expressed genes. Also the data will be mined to explore the development of molecular markers (SSRs and SNPs). For this, a sugarcane cultivar will be submitted to photoperiod treatments of flowering induction and non-induction (control) in an automated Photoperiod facility in a period comprising the beginning of induction until flowering. The sampling time point corresponding to the change of the vegetative to reproductive stage will be selected to be used for the RNA-seq analysis. We emphasize that, to our knowledge, to date, the transcriptome of sugarcane during flowering induction, under controlled photoperiod conditions has not yet been investigated, characterizing the proposal as of great potential to unravel important sugarcane flowering genes and hence contribute for the agricultural bio-economy of the country. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA SANTOS, PAULO H.; VIEIRA MANECHINI, JOAO R.; BRITO, MICHAEL S.; ROMANEL, ELISSON; VICENTINI, RENATO; SCARPARI, MAXIMILIANO; JACKSON, STEPHEN; PINTO, LUCIANA R.. Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.). SCIENTIFIC REPORTS, v. 11, n. 1, FEB 25 2021. (18/08380-0, 16/02679-9)

VENAIL, JULIEN; DA SILVA SANTOS, PAOLO HENRIQUE; MANECHINI, JOAO RICARDO; ALVES, LEONARDO CARDOSOS; SCARPARI, MAXIMILIANO; FALCAO, THAIS; ROMANEL, ELISSON; BRITO, MICHAEL; VICENTINI, RENATO; PINTO, LUCIA; et al. Analysis of the PEBP gene family and identification of a novel FLOWERING LOCUS T orthologue in sugarcane. Journal of Experimental Botany, v. 73, n. 7, p. 15-pg., 2022-01-21. (16/02679-9, 18/08380-0)

MANECHINI, JOAO RICARDO VIEIRA; SANTOS, PAULO HENRIQUE DA SILVA; ROMANEL, ELISSON; BRITO, MICHAEL DOS SANTOS; SCARPARI, MAXIMILIANO SALLES; JACKSON, STEPHEN; PINTO, LUCIANA ROSSINI; VICENTINI, RENATO. Transcriptomic Analysis of Changes in Gene Expression During Flowering Induction in Sugarcane Under Controlled Photoperiodic Conditions. FRONTIERS IN PLANT SCIENCE, v. 12, JUN 15 2021. (18/08380-0, 16/02679-9)