Classical and molecular markers for chromosomal studies in anurans

• Grant number: 16/07717-6
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: February 28, 2019
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Shirlei Maria Recco-Pimentel
• Grantee: Shirlei Maria Recco-Pimentel
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Daniel Pacheco Bruschi

Abstract

Although only a few anurans had their chromosomes investigated up to now, the data yet obtained are exciting because it helps to clarify the mechanisms of karyotypic evolution. Classical, and more recently molecular markers have been used to investigate the chromosomal variation in anurans, improving the comparative cytogenetics. In the present work, the karyotypes of Phyllomedusa (sub-project 1) and Pipa (sub-project 2) species will be studied. Phyllomedusa ayeaye, P.megacephala e P. palliata will be analyzed through classical cytogenetics aiming to contribute to the understanding of karyotype evolution in Phyllomedusa genus. Cytogenomic analyses are proposed aiming to obtain new chromosomal markers to detect chromosomes rearrangements, to understand the intrageneric variation of diploid number (2n=20, 22 e 30) in the genus Pipa. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DANIEL PACHECO BRUSCHI; DEBORAH YASMIM SOUSA; AMANDA SOARES; KLÉLIA APARECIDA DE CARVALHO; CARMEN SÍLVIA BUSIN; NATÁLIA CRISTINE FICANHA; ALBERTINA PIMENTEL LIMA; GILDA VASCONCELLOS ANDRADE; SHIRLEI MARIA RECCO-PIMENTEL. Comparative cytogenetics of nine populations of the Rhinella genus (Anura, Bufonidae) with a highlight on their conservative karyotype. GENETICS AND MOLECULAR BIOLOGY, v. 42, n. 2, p. 445-451, 2019-06-27. (16/07717-6)

JULIA R. ERNETTI; CAMILLA B. GAZOLLA; SHIRLEI M. RECCO-PIMENTEL; ELAINE M. LUCA; DANIEL P. BRUSCHI. Non-random distribution of microsatellite motifs and (TTAGGG)n repeats in the monkey frog Pithecopus rusticus (Anura, Phyllomedusidae) karyotype. GENETICS AND MOLECULAR BIOLOGY, v. 42, n. 4, 2020-01-13. (16/07717-6)

ZATTERA, MICHELLE LOUISE; GAZOLLA, CAMILLA BORGES; SOARES, AMANDA DE ARAUJO; GAZONI, THIAGO; POLLET, NICOLAS; RECCO-PIMENTEL, SHIRLEI MARIA; BRUSCHI, DANIEL PACHECO. Evolutionary Dynamics of the Repetitive DNA in the Karyotypes of Pipa carvalhoi and Xenopus tropicalis (Anura, Pipidae). FRONTIERS IN GENETICS, v. 11, JUL 21 2020. (16/07717-6)

ZATTERA, MICHELLE LOUISE; LIMA, LUANA; DUARTE, IRAINE; DE SOUSA, DEBORAH YASMIN; DOS SANTOS ARAUJO, OLIVIA GABRIELA; GAZONI, THIAGO; MOTT, TAMI; RECCO-PIMENTEL, SHIRLEI MARIA; BRUSCHI, DANIEL PACHECO. Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype. COMPARATIVE CYTOGENETICS, v. 13, n. 3, p. 297-309, OCT 14 2019. (16/07717-6)

GAMA, JOANA MOURA; GAZOLLA, CAMILLA BORGES; DE SOUZA, DEBORAH YASMIN; RECCO-PIMENTEL, SHIRLEI MARIA; BRUSCHI, DANIEL PACHECO. Recurrent variation in the active NOR sites in the monkey frogs of the genus Pithecopus Cope, 1866 (Phyllomedusidae, Anura). COMPARATIVE CYTOGENETICS, v. 13, n. 4, p. 325-338, OCT 21 2019. (16/07717-6)