Parity, ovarian development, vertical transmission and locomotor activity of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in the city of São Paulo -SP.

• Grant number: 16/12140-0
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2016 - October 31, 2018
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Tamara Nunes de Lima Camara
• Grantee: Tamara Nunes de Lima Camara
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Mauro Toledo Marrelli

Abstract

Ae. aegypti is considered the main vector of dengue, chikungunya and Zika viruses in Brazil. In addition, Aedes albopictus is considered a potential vector of these three arboviruses. The longevity of mosquito vectors is closely related to the parity of the female, which can be determined by observing the tracheiolas which oxygenate ovaries, whereas gonotrophic discordance is related to ovarian development and the blood contained in the stomach of females. The presence of vertical transmission in populations of mosquito vectors is of great epidemiological importance, as it allows the virus circulation maintenance in inter-epidemic periods. Competition between immature forms of Ae. aegypti and Ae. albopictus may generate smaller adults, which may affect some aspects of their biology, such as activity. The objective of this project is to evaluate the parity and ovarian development of Ae. aegypti and Ae. albopictus collected at USP Sport Park - University City and at the School of Public Health of USP, as well as to detect dengue virus in males and females of these species, originated from eggs collected in the Municipal Piqueri Park, East Zone of São Paulo. In addition, we will assess the effect of intraspecific competition on the locomotor activity of females of both species. The stomach and ovaries of Ae. aegypti and Ae. albopictus females will be dissected. The isolation of DENV will be done by RT-PCR. For locomotor activity, under laboratory conditions, virgin females emerged from artificial containers with low and high intraspecific competition will be evaluated with specific activity monitors. This study will evaluate some important parameters in these two important vector species in Brazil in order to better understand the dynamics of these mosquito populations and direct control strategies. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA-DE-LIMA, VICTOR HENRIQUE; ANDRADE, PAMELA DOS SANTOS; THOMAZELLI, LUCIANO MATSUMIYA; MARRELLI, MAURO TOLEDO; URBINATTI, PAULO ROBERTO; MARQUES DE SA ALMEIDA, ROSA MARIA; LIMA-CAMARA, TAMARA NUNES. Silent circulation of dengue virus in Aedes albopictus (Diptera: Culicidae) resulting from natural vertical transmission. SCIENTIFIC REPORTS, v. 10, n. 1, MAR 2 2020. (17/12434-6, 16/12140-0)

FERREIRA-DE-LIMA, VICTOR HENRIQUE; PORTELA CAMARA, DANIEL CARDOSO; HONORIO, NILDIMAR ALVES; LIMA-CAMARA, TAMARA NUNES. The Asian tiger mosquito in Brazil: Observations on biology and ecological interactions since its first detection in 1986. Acta Tropica, v. 205, MAY 2020. (16/12140-0)

FERREIRA-DE-LIMA, VICTOR HENRIQUE; LIMA-CAMARA, TAMARA NUNES. Natural vertical transmission of dengue virus in Aedes aegypti and Aedes albopictus: a systematic review. PARASITES & VECTORS, v. 11, FEB 1 2018. (16/12140-0, 17/12434-6)

LIMA-CAMARA, TAMARA NUNES; MEDEIROS-SOUSA, ANTONIO RALPH; COELHO, RONAN ROCHA; MARRELLI, MAURO TOLEDO. Body size does not affect locomotor activity of Aedes aegypti and Aedes albopictus females (Diptera:Culicidae). Acta Tropica, v. 231, p. 8-pg., 2022-07-01. (16/12140-0)

NAKAZATO, BRUNO MAGALHAES; DA GRACA MACORIS, MARIA DE LOURDES; URBINATTI, PAULO ROBERTO; LIMA-CAMARA, TAMARA NUNES. Locomotor activity in Aedes aegypti with different insecticide resistance profiles. Revista de Saúde Pública, v. 55, 2021. (16/12140-0)

SABRINA RIBEIRO SANTANA; PÂMELA DOS SANTOS ANDRADE; PAULO ROBERTO URBINATTI; ROSA MARIA MARQUES DE SÁ ALMEIDA; TAMARA NUNES LIMA-CAMARA. Gonotrophic discordance in Culex quinquefasciatus (Diptera: Culicidae) in the city of São Paulo, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, 2019-12-20. (16/12140-0)