SYNTHESIS AND CHARACTERIZATION OF AN EXPERIMENTAL DENTAL CEMENT DERIVED FROM EXTRACT OF RED SEAWEEDS

• Grant number: 16/07996-2
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2016
• End date: September 30, 2018
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Marcela Rocha de Oliveira Carrilho
• Grantee: Marcela Rocha de Oliveira Carrilho
• Host Institution: Centro Universitário Anhanguera de São Paulo. Anhanguera Educacional Participações S/A. São Bernardo do Campo , SP, Brazil
• Associated researchers: Ana Paula Dias Ribeiro ; Fabiana Barbara Piveta ; Fernanda Cristina Pimentel Garcia ; Nilana Meza Tenório de Barros Jaggi ; Susana Nogueira Diniz

Abstract

Natural products of marine origin feature a variety of bioactive component and, thus, represent a source of interest in the search for new drugs. In order to survive competition, algae and other marine organisms develop defense mechanisms that result in the formation of a variety of secondary metabolites. A growing number of isolated substances from such organisms have their chemical structure evaluated and assessed against different biological targets, helping to expand knowledge in the field of bioactive natural products. The object of study of this research is to explore the dental therapeutic potential of a species of red algae that we will call AlgaeExp, because it has been protected as intellectual product which the potential to become a patent has been under preliminary analysis. Brazil has one of the largest resources of such algae, a renewable source of minerals that has been marketed in the country and abroad as a nutritional supplement, especially as a calcium repository. Supplements and/or AlgaeExp extracts have been used successfully, for example, in stimulating bone formation, consolidation of failures in cortical bone, in long bone growth and calcium bioavailability in this tissue. Preliminary studies performed in our laboratory with a solution of AlgaExp extract showed a partial remineralization of enamel (similar to that obtained with fluorides) after it has been subjected to in vitro and in situ/ex vivo acidogenic challenges (unpublished data). In a pilot study we could prepared and tested, preliminarily, a mixture of water and AlgaExp extract that resulted in a type of cement with handling characteristics (working time and prey) and flow that are in accordance with those normalized by ISO (International Standards Organization) for this class of biomaterial (water-based dental cement - unpublished data). The aim of this research project is to formulate and characterize this experimental cement, exploring its bioactive potential in stimulating, for instance, new formation of dentin and bone. Physical, mechanical, chemical and biological properties of this experimental cement will be evaluated by techniques described in details in the body of the proposal. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARRILHO, MARCELA R.; SCAFFA, POLLIANA M. C.; DIONIZIO, ALINE; VENTURA, TALITA M. O.; BUZALAF, MARILIA A. R.; VIDAL, CRISTINA M. P.. Differential analysis of the dentin soluble proteomic. Journal of Dentistry, v. 131, p. 10-pg., 2023-02-24. (16/07996-2, 14/12316-5, 11/12226-8)

CARRILHO, MARCELA R.; SCAFFA, POLLIANA; OLIVEIRA, VITOR; TJADERHANE, LEO; TERSARIOL, IVARNE L.; PASHLEY, DAVID H.; TAY, FRANKLIN; NASCIMENTO, FABIO D.. Insights into cathepsin-B activity in mature dentin matrix. ARCHIVES OF ORAL BIOLOGY, v. 117, SEP 2020. (16/07996-2, 13/05822-9, 11/12226-8)