OBSERVING THE INTRACELLULAR pH OF MICROGLIAL CELLS

• Grant number: 16/17951-6
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2016
• End date: November 30, 2018
• Field of knowledge: Biological Sciences - Biophysics - Cellular Biophysics
• Principal Investigator: Manoel de Arcisio Miranda Filho
• Grantee: Manoel de Arcisio Miranda Filho
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

This project has as main objective to obtain dynamic images of changes in intracellular pH (pHi) in microglia cells. Unlike other pHi measurement methods, the use of fluorescence microscopy will provide us with simultaneously spatial and temporal resolution of the events involved in maintaining the microglial pHi. Three majors will be studied: 1) the relationship between the composition of the plasma membrane and regulation of pHi; 2) the relationship between metabolic condition of hypoglycemia and hyperglycemia and regulation of pHi; and 3) the molecular mechanism for the intracellular trafficking of proteins involved in the regulation of the pHi of these cells. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, VITOR S.; ALVES-SILVA, HELIO S.; ORTS, DIEGO J. B.; RIBEIRO-SILVA, LUISA; ARCISIO-MIRANDA, MANOEL; OLIVEIRA, FERNANDO A.. Calcium Signaling in Neurons and Glial Cells: Role of Cav1 channels. Neuroscience, v. 421, p. 95-111, NOV 21 2019. (12/50336-2, 16/17951-6, 16/50484-2)

ORTS, DIEGO J. B.; PEIGNEUR, STEVE; SILVA-GONCALVES, LAIZ COSTA; ARCISIO-MIRANDA, MANOEL; BICUDO, JOSE EDUARDO P. W.; TYTGAT, JAN. AbeTx1 Is a Novel Sea Anemone Toxin with a Dual Mechanism of Action on Shaker-Type K+ Channels Activation. MARINE DRUGS, v. 16, n. 10, OCT 2018. (16/13368-4, 16/17951-6, 09/07128-7, 11/21031-6)

ALVES, VITOR S.; ARCISIO-MIRANDA, MANOEL; CARRETTIERO, DANIEL C.; OLIVEIRA, FERNANDO A.. Transport of Glucose by the Plasma Membrane Affects the Removal and Concentration of Ca2+ at Rest in Neurons - Implications of a Condition Prior to Alzheimer's Disease?. Neuroscience, v. 431, p. 52-63, APR 1 2020. (12/50336-2, 16/17951-6, 16/50484-2)

ORTS, DIEGO J. B.; ARCISIO-MIRANDA, MANOEL. Cell glycosaminoglycans content modulates human voltage-gated proton channel (H(V)1) gating. FEBS Journal, NOV 2021. (16/17951-6, 17/25204-9, 16/13368-4)

MARTINS, DANUBIA BATISTA; PACCA, CAROLINA COLOMBELLI; DA SILVA, ANNIELLE MENDES BRITO; DE SOUZA, BIBIANA MONSON; DE ALMEIDA, MARGARETE TERESA GOTTARDO; PALMA, MARIO SERGIO; ARCISIO-MIRANDA, MANOEL; DOS SANTOS CABRERA, MARCIA PEREZ. Comparing activity, toxicity and model membrane interactions of Jelleine-I and Trp/Arg analogs: analysis of peptide aggregation. Amino Acids, v. 52, n. 5, MAY 2020. (16/16212-5, 16/13368-4, 16/17951-6, 14/08372-7, 12/24259-0)