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Occurrence of Mycobacterium spp and Francisella spp in granulomatous disease of fish in aquaculture: stock breeding and São Paulo wild. implementation of diagnostic techniques

Grant number: 16/12930-0
Support type:Regular Research Grants
Duration: November 01, 2016 - April 30, 2019
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering
Principal Investigator:Ana Maria Cristina Rebello Pinto da Fonseca Martins
Grantee:Ana Maria Cristina Rebello Pinto da Fonseca Martins
Home Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
Assoc. researchers:Danielle de Carla Dias ; Marcia Helena Braga Catroxo ; Marcio Hipolito

Abstract

This project will include the study of granulomatous diseases, caused byMycobacterium spp and Francisella spp bacteria, which have serious implications onAnimal Health, Public Health and Agribusiness. There are few studies in our fieldaimed at their detection and characterization in a greater extent. Diagnostic techniqueswill be implemented to verify the occurrence of these bacteria in fish farms (study 1),wild freshwater animals in the State of São Paulo (study 2) and materials stored in ourlaboratory (study 3). To this purpose, we intend to determine the occurrence ofcirculating strains (?) of these bacteria, starting from organ fragments deriving fromstudy 1, study 2 and study 3 of the State of São Paulo, through the use of "in situ"hybridization reaction, immunohistochemistry, optical microscopy (hematoxylin-eosin,and Ziehl Nielsen or Fite-Faraco), electron microscopy (Negative staining andimmunostaining with colloidal gold). The results will contribute to the understanding ofthe epidemiology of granulomatous fish diseases, particularly as regards the design ofmore effective preventive measures, such as immunoprophylaxis, improvingsurveillance and providing information about their relationships with other animalspecies. (AU)