Occurrence of Mycobacterium spp. and Francisella spp. in granulomatous disease of fish in aquaculture: stock and wild breeding in São Paulo: implementation of diagnostic techniques

• Grant number: 16/12930-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2016
• End date: April 30, 2019
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Ana Maria Cristina Rebello Pinto da Fonseca Martins
• Grantee: Ana Maria Cristina Rebello Pinto da Fonseca Martins
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Danielle de Carla Dias ; Marcia Helena Braga Catroxo ; Marcio Hipolito

Abstract

This project will include the study of granulomatous diseases, caused by Mycobacterium spp. and Francisella spp. bacteria, which have serious implications on Animal Health, Public Health and Agribusiness. There are few studies in our field aimed at their detection and characterization in a greater extent. Diagnostic techniques will be implemented to verify the occurrence of these bacteria in fish farms (study 1), wild freshwater animals in the State of São Paulo (study 2) and materials stored in our laboratory (study 3). To this purpose, we intend to determine the occurrence of circulating strains (?) of these bacteria, starting from organ fragments deriving from study 1, study 2 and study 3 of the State of São Paulo, through the use of "in situ" hybridization reaction, immunohistochemistry, optical microscopy (hematoxylin-eosin,and Ziehl Nielsen or Fite-Faraco), electron microscopy (Negative staining and immunostaining with colloidal gold). The results will contribute to the understanding of the epidemiology of granulomatous fish diseases, particularly as regards the design of more effective preventive measures, such as immunoprophylaxis, improving surveillance and providing information about their relationships with other animal species. (AU)