Gene expression analysis on in vitro neuronal models and post-mortem brain tissues to understand Autism Spectrum Disorder

• Grant number: 16/50324-5
• Support type: Regular Research Grants
• Duration: May 01, 2017 - April 30, 2019
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Cooperation agreement: University of New South Wales
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal researcher: Andréa Laurato Sertié
• Grantee: Andréa Laurato Sertié
• Principal researcher abroad: Irina Voineagu
• Institution abroad: University of New South Wales (UNSW), Australia
• Home Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil
• Associated research grant: 15/50138-4 - Elucidating the role of the innate immune complement pathway in brain development and in Autism Spectrum Disorders, AP.R

Abstract

We propose to combine the complementary expertise of our labs in cellular reprogramming and exome sequencing (Sertié's group) and transcriptome sequencing co-expression network reconstruction (Voineagu lab) to investigate convergent pathophysiology of non-syndromic ASD and Rett syndrome. Dr. Sertie's group is studying the possible role of the complement system in the etiology of ASD, through the analysis of iPSC-derived neuronal cells from patients harbouring variants in genes related to this system. Dr. Voineagu's lab has been studying transcriptome changes in human brain tissue of Rett syndrome cases and identified an upregulation of genes involved in the complement pathway. Thus, through a series of exchange activities, we aim to share our different expertise's and data, which will enable a deeper investigation of the specific questions of each proponent, it will contribute for other ongoing projects in both labs, thereby helping to create basis for a long term partnership. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRIESI-OLIVEIRA, K.; FOGO, M. S.; PINTO, B. G. G.; ALVES, A. Y.; SUZUKI, A. M.; MORALES, A. G.; EZQUINA, S.; SOSA, O. J.; SUTTON, G. J.; SUNAGA-FRANZE, D. Y.; et al. Transcriptome of iPSC-derived neuronal cells reveals a module of co-expressed genes consistently associated with autism spectrum disorder. MOLECULAR PSYCHIATRY, v. 26, n. 5, p. 1589-1605, MAY 2021. (13/08028-1, 16/50324-5, 14/10068-4)