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Photosensitizers aggregation and its consequences in photochemical mechanisms and Photodynamic Therapy effectivity

Grant number: 16/03037-0
Support type:Regular Research Grants
Duration: February 01, 2017 - August 31, 2019
Field of knowledge:Biological Sciences - Biophysics - Radiology and Photobiology
Principal Investigator:Christiane Pavani
Grantee:Christiane Pavani
Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
Assoc. researchers:Anna Carolina Ratto Tempestini Horliana ; Daniela de Fátima Teixeira da Silva ; Gabriela Alves da Collina ; Simone Aquino


Photodynamic Therapy (PDT) is a treatment modality that have been showing to be promising in many health sciences, such as dentistry and medicine, being applied at microorganisms inactivation and tumor treatment. Due to its low cost, methylene blue (MB), which is a phenothiazine photosensitizer (PS), it has been extensively known in Brazil. Other phenothiazines, such as Azure A, Azure B and Dimethylmethylene blue, can be interesting PSs due to their structure similar to MB. Literature regarding MB effectivity are conflicting. Since MB presents peculiar physico-chemical properties. Therefore, the identification of the relationships between aggregation, action mechanism and effectivity against microorganisms, as well as the establishment of new media and formulas that can favor the most effective mechanism, is essential. This work includes solution, Candida albicans planktonic culture and biofilms studies to evaluate the MB aggregation effect on the effectivity of antimicrobial PDT, with the aim to stablish the most effective photochemical mechanism, besides evaluating the other phenothiazines action. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA COLLINA, GABRIELA ALVES; FREIRE, FERNANDA; BARBOSA, VINICIUS DA SILVA; CORREA, CAROLINE BENTO; NASCIMENTO, HELENYCE REIS; RATTO TEMPESTINI HORLIANA, ANNA CAROLINA; TEIXEIRA DA SILVA, DANIELA DE FATIMA; PRATES, RENATO ARAUJO; PAVANI, CHRISTIANE. Photodynamic antimicrobial chemotherapy action of phenothiazinium dyes in planktonic Candida albicans is increased in sodium dodecyl sulfate. Photodiagnosis and Photodynamic Therapy, v. 29, MAR 2020. Web of Science Citations: 0.
DA COLLINA, GABRIELA ALVES; FREIRE, FERNANDA; DA COSTA SANTOS, THABATA PAULINO; SOBRINHO, NATALIA GESSE; AQUINO, SIMONE; PRATES, RENATO ARAUJO; TEIXEIRA DA SILVA, DANIELA DE FATIMA; RATTO TEMPESTINI HORLIANA, ANNA CAROLINA; PAVANI, CHRISTIANE. Controlling methylene blue aggregation: a more efficient alternative to treat Candida albicans infections using photodynamic therapy. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 17, n. 10, p. 1355-1364, OCT 1 2018. Web of Science Citations: 5.
FRANCISCO, CLAUDIA M. L.; GONCALVES, JOSE M. L. A.; BRUM, BRUNO S.; SANTOS, THABATA P. C.; LINO-DOS-SANTOS-FRANCO, ADRIANA; SILVA, DANIELA F. T.; PAVANI, CHRISTIANE. The photodynamic efficiency of phenothiazinium dyes is aggregation dependent. NEW JOURNAL OF CHEMISTRY, v. 41, n. 23, p. 14438-14443, DEC 7 2017. Web of Science Citations: 4.

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