Determination of the metabolomic profile of non-Hodgkin lymphoma for the identification of tumor biomarkers

• Grant number: 16/07014-5
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: July 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Ana Valéria Colnaghi Simionato
• Grantee: Ana Valéria Colnaghi Simionato
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Marcos Nogueira Eberlin ; Ronei Jesus Poppi ; Victor Piana de Andrade ; Vladmir Cláudio Cordeiro de Lima

Abstract

Non Hodgkin lymphoma prognostic has been achieved by the International Prognostic Index lately, which is obtained from physical characteristics, pathological observations and symptoms reported by the patient. Therefore, searching for prognostic and prediction tumor biomarkers for non Hodgkin lymphoma is crucial, aiming to preview more accurately and earlier the disease progress as well as the suitable treatment to each subject. The metabolomic profile of non Hodgkin lymphoma will be investigated by LC-MS/MS, GC-MS and CE-MS/MS in this project, so that prognostic and prediction biomarkers may be identified. Firstly, blood serum and urine samples from healthy subjects will be analyzed by LC-MS/MS, GC-MS and CE-MS/MS for optimization of analyses conditions. Sample treatment for LC-MS/MS and CE-MS/MS will be as simple as possible, so that analytes loss is circumvented. On the other hand, sample treatment for GC-MS will require more extensive biological sample manipulation in order to obtain volatile and or more thermally stable analytes. For this purpose, three procedures will be evaluated: conventional derivatization; volatile organic compounds analyses; on fiber solid phase microextraction derivatization. Concomitantly, blood serum and urine samples from non Hodgkin lymphoma subjects will be collected and stored before each chemotherapy cycle, and will be analyzed by the developed methods. After data treatment by multivariate statistics, the potential biomarkers will be validated and will possibly aid on the treatment to be used for each subject by a custom medicine approach. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BUENO DUARTE, GUSTAVO HENRIQUE; ALVES DE PILOTO FERNANDES, ANNA MARIA; ROSINI SILVA, ALEX APARECIDO; ZAMORA-OBANDO, HANS R.; AMARAL, ALAN GONCALVES; MESQUITA, ALESSANDRA DE SOUSA; SCHMIDT-FILHO, JAYR; CORDEIRO DE LIMA, VLADMIR C.; COSTA, FELIPE D'ALMEIDA; ANDRADE, VICTOR PIANA; et al. Gas chromatography-mass spectrometry untargeted profiling of non-Hodgkin's lymphoma urinary metabolite markers. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SEP 2020. (16/07014-5)

MESQUITA, ALESSANDRA DE SOUSA; ZAMORA-OBANDO, HANS ROLANDO; DOS SANTOS, FABIO NEVES; SCHMIDT-FILHO, JAYR; DE LIMA, VLADMIR CORDEIRO; COSTA, FELIPE D'ALMEIDA; DE ANDRADE, VICTOR PIANA; EBERLIN, MARCOS NOGUEIRA; COLNAGHI SIMIONATO, ANA VALERIA. Volatile organic compounds analysis optimization and biomarker discovery in urine of Non-Hodgkin lymphoma patients before and during chemotherapy. Microchemical Journal, v. 159, DEC 2020. (16/07014-5, 17/09726-5)

BUENO DUARTE, GUSTAVO HENRIQUE; ALVES DE PILOTO FERNANDES, ANNA MARIA; ROSINI SILVA, ALEX APARECIDO; ZAMORA-OBANDO, HANS R.; AMARAL, ALAN GONCALVES; MESQUITA, ALESSANDRA DE SOUSA; SCHMIDT-FILHO, JAYR; CORDEIRO DE LIMA, VLADMIR C.; COSTA, FELIPE D'ALMEIDA; ANDRADE, VICTOR PIANA; et al. Gas chromatography-mass spectrometry untargeted profiling of non-Hodgkin's lymphoma urinary metabolite markers. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 412, n. 27, p. 12-pg., 2020-09-08. (16/07014-5)