Role of hyperbaric oxygen therapy in the control of brain lesions in experimental hydrocephalus

• Grant number: 16/11212-7
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Luiza da Silva Lopes
• Grantee: Luiza da Silva Lopes
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Helio Rubens Machado ; Omar Feres
• Associated scholarship(s): 18/01222-0 - Role of hyperbaric oxygen therapy in the control of brain lesions in experimental hydrocephalus, BP.TT

Abstract

Diseases affecting the central nervous system (CNS) can produce disastrous consequences, especially when they reach the developing brain. These results imply dysfunction not only to the affected individuals, but also lead to emotional and financial disruption to families and caregivers, and mean costs to the health system. Hydrocephalus is one of the most common and complex neurological conditions in the clinical practice, and can be defined as a change from liquid flow of cerebrospinal fluid (CSF) within the ventricles. The commonly used treatment of hydrocephalus is surgical, usually through the shunt. However, as not all patients can be treated surgically immediately after diagnosis, neuroprotection proceedings have been studied. With the increase of CSF volume, encephalic tissues are subjected to compression and stretching forces, leading to blood flow fall and ischemia, with consequent reduction in oxygenation of surrounding tissues. The aim of this study is verify whether hyperbaric oxygen therapy applied in hydrocephalic rats can offer benefits to the structures affected by ventriculomegaly. They will be used seven-day-old Wistar rats submitted to hydrocephalus by intracisternal injection of 10% kaolin. The animals will be divided into four groups: controls (n = 10), controls treated with hyperbaric oxygen therapy (2 ATA / 2h / day) from the 8th day of life (n = 10), hydrocephalic rats untreated (n = 10), hydrocephalic rats treated with hyperbaric oxygen (2 ATA / 2h / day), from day 1 post induction (eight day-old) (n = 10). To evaluate the response to treatment are behavioral tests (open field, automatic monitor activities, object recognition tasks, and Morris water maze), histological immunohistochemical studies (anti-GFAP, -Ki67, -VEGF, -COX-2 e -HIF1-Alpha) and proteins quantification by ELISA (GFAP, MBP, VEGF, COX-2 e HIF1-Alpha). The results may indicate if the hyperbaric oxygen therapy exhibits some potential to be used as a complement treatment in the hydrocephalus. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROMEIRO, THAIS HELENA; DA SILVA, STEPHANYA COVAS; BEGGIORA, PAMELLA DA SILVA; SAMPAIO, GUSTAVO BOTELHO; BRANDAO, RICARDO ANDRADE; SANTOS, MARCELO VOLPON; MACHADO, HELIO RUBENS; LOPES, LUIZA DA SILVA. he association of Edaravone with shunt surgery improves behavioral performance, reduces astrocyte reaction and apoptosis, and promotes neuroprotection in young hydrocephalic rat. Journal of Chemical Neuroanatomy, v. 119, JAN 2022. (16/11212-7)

PINTO DE MOURA SILVA, GABRIEL APARECIDO; DA SILVA, STEPHANYA COVAS; BEGGIORA, PAMELLA DA SILVA; MATIAS JUNIOR, IVAIR; MENEZES-REIS, RAFAEL; SANTOS, MARCELO VOLPON; MACHADO, HELIO RUBENS; LOPES, LUIZA DA SILVA. Transcranial ultrasonography as a reliable instrument for the measurement of the cerebral ventricles in rats with experimental hydrocephalus: a pilot study. CHILD'S NERVOUS SYSTEM, v. 37, n. 6, FEB 2021. (09/54010-1, 16/11212-7)

DA SILVA, STEPHANYA COVAS; FERES, OMAR; BEGGIORA, PAMELLA DA SILVA; MACHADO, HELIO RUBENS; MENEZES-REIS, RAFAEL; ARAUJO, JOAO EDUARDO; BRANDAO, RICARDO ANDRADE; LOPES, LUIZA DA SILVA. Hyperbaric oxygen therapy reduces astrogliosis and helps to recovery brain damage in hydrocephalic young rats. CHILD'S NERVOUS SYSTEM, v. 34, n. 6, p. 1125-1134, JUN 2018. (13/04130-6, 16/11212-7)

ROCHA CATALAO, CARLOS HENRIQUE; SOUZA, ANDERSON OLIVEIRA; SANTOS-JUNIOR, NILTON NASCIMENTO; DA SILVA, STEPHANYA COVAS; ANGENENDT DA COSTA, LUIS HENRIQUE; ALBERICI, LUCIANE CARLA; ALVES ROCHA, MARIA JOSE; LOPES, LUIZA DA SILVA. Kaolin-induced hydrocephalus causes acetylcholinesterase activity dysfunction following hypothalamic damage in infant rats. Brain Research, v. 1724, DEC 1 2019. (16/23509-4, 16/11212-7)