Morfometric and gene expression of experimentally induced wounds in rabbits and treated with biomaterials and rosuvastatin

• Grant number: 16/25938-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2017
• End date: September 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Cecília Laposy Santarém
• Grantee: Cecília Laposy Santarém
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil
• Associated researchers: Anthony César de Souza Castilho ; Luis Antonio Justulin Junior

Abstract

Recent advanced in development and modification of biomaterials pointed out the platelet concentrates (PRP/PRF, respectively, platelet-rich plasm and platelet-rich fibrin) as promising biomaterials to be employed in regenerative medicine. Furthermore, experimental studies have been shown the pleiotropic effects of statins, particularly the anti-inflammatory, antioxidant and immunomodulating activities; the cellular proliferation and the action on the endothelial protection. Thus, the aim of this study is to evaluate the anti-inflammatory and cicatrizing effects of autologus PRP or PRF + rosuvastatin (R) association on the experimentally induced wound healing in rabbits. Sixteen New Zealand white male adult rabbits will be submitted to a surgical procedure using an 8.0 mm punch in order to induce four wound healing in the dorsal region (two wounds in the left and two in the right side). Following, two groups (n= 8 each) will be formed. In group 1 (G1), each wound will be treated with a different substance: PRP, R and PRP+R. In group 2 (G2), each wound will be treated with: PRF, R and PRF+R. In both two experimental groups one wound will serve as control (NaCl 0.9% solution). After surgery, wounds will be covered with a ryon sterile and an adhesive curative (Band AidÒ). On the 17th day post-surgery the scar tissue will be analyzed for classification and quantification of collagen fibers. The computer program Image J will be used to evaluate the reduction in the area of the wounds, while for determining the type of collagen, as well as its quantification, biopsy slides will be stained and passed through the polarization technique using Picrosirius-red F3BA dye. RNA extraction will be performed in order to evaluate the gene expression of the collagen. Genes will be quantified by a real-time PCR assay. The SPSS computer program will be used for all statistical analysis, adopting a 5% significance level. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TETILA, AMANDA FEBA; SANTOS BREDA, MARIA ROSA; BARILLI NOGUEIRA, ROSA MARIA; NAI, GISELE ALBORGHETTI; LAPOSY, CECILIA BRAGA. The Use of Platelet-Rich Plasma and Rosuvastatin in Wound Healing in Rabbits: A Longitudinal Study. ADVANCES IN SKIN & WOUND CARE, v. 32, n. 9, p. 1-5, SEP 2019. (16/25938-0)