Evaluation of platelet concentrations (platelet-rich plasma and platelet-rich fibrin) in the treatment of experimentally induced surgical wounds in rabbits

• Grant number: 15/14713-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2015
• Effective date (End): September 30, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Cecília Laposy Santarém
• Grantee: Maria Rosa Santos Breda
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

The healing process comprises a sequence of molecular and cellular events that interact so that the restoration of damaged tissue can occur properly. In recent years, scientific advances have been made in evaluating the effects of using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) on the hemostatic process and for wound healing through the release of growth factors. Comparing the effectiveness of both platelet preparations (PRP and FRP), studies have suggested that FRP is more effective that PRP and consider it a promising option in the treatment of wounds. However, studies on the effectiveness of FRP compared to PRP, in experimental models involving rabbits, in terms of analysis of the healing quality provided by the two techniques, is scarce in the literature. The proposed study aims to characterize the type of collagen fiber induced by dermal wounds in rabbits treated with PRP (gel) and autologous FRP. Twelve clinically healthy rabbits of the New Zealand breed (n = 6 males, n = 6 females) will be used. After the anesthetic procedure, two surgical wounds will be induced in the dorsal region, with the help of an 8.00 mm punch. Six animals will be treated with autologous PRP, in gel form, and the other six rabbits with PRF, in one of wounds. The second wound, parallel to the first, will be treated with saline solution and serve as a control. The computer program Image J will be used to evaluate the reduction in the area of the wounds, while for determining the type of collagen, as well as its quantification, biopsy slides will be stained and passed through the polarization technique using Picrosirius-red F3BA dye. The SPSS computer program will be used for all statistical analysis, adopting a 5% significance level.