Evaluation of inflammation and angiogenesis in axial pattern flaps toracodorsal associated wtih the use of rich plasma in platelets in rabbits (Oryctolagus cuniculus)

• Grant number: 17/24628-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2018
• End date: September 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Andrigo Barboza de Nardi
• Grantee: Caroline Yuri Kajiura
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

When tissues lose their viability, there is the possibility of repairing it using reconstructive surgery. For this, flaps or grafts may be used. The flaps consist of a portion of tissue partially removed from the site of origin and transferred to its receptor bed, having a base which allows the blood supply to sustain the viability of the flap. The grafts consist of the total withdrawal of the skin segment transferred to a distant recipient bed, being more susceptible to ischemia and necrosis after implantation, because they are not irrigated by the brachial or subdermal plexus. In order to be successful after a surgical procedure, Platelet-Rich Plasma (PRP) can be used, a product capable of promoting healing due to growth factors (HR), stimulating angiogenesis and reducing the risk of postoperative necrosis . The objective of this study was to evaluate the inflammation (presence of inflammatory cells) and angiogenesis (vascular endothelial analysis) in samples of cutaneous flaps of the thoracodorsal axial pattern associated with the use of platelet-rich plasma. The inflammation will be evaluated by the histochemical technique by counting mononucleated and polymorphonucleated cells. In addition, re-epithelialization, fibroblast proliferation, hemorrhage and the presence or absence of necrotic areas in histological sections stained by Hematoxylin & Eosin (HE) will be evaluated. Angiogenesis will be evaluated by the immunohistochemical technique by counting immunoreactive cells using monoclonal antibodies (CD-31).