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Sensorimotor recovery following spinal root axotomy: use of different experimental approaches

Grant number: 18/05006-0
Support type:Research Projects - Thematic Grants
Duration: February 01, 2019 - January 31, 2024
Field of knowledge:Biological Sciences - Morphology
Principal Investigator:Alexandre Leite Rodrigues de Oliveira
Grantee:Alexandre Leite Rodrigues de Oliveira
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers:Ângela Cristina Malheiros Luzo ; Benedito Barraviera ; Cecilia Amelia de Carvalho Zavaglia ; Frank Kirchhoff ; Maria Helena Andrade Santana ; Rui Seabra Ferreira Junior
Associated grant(s):18/17554-2 - Multi-User Equipment approved in grant 2018/05006-0: flow cytometer - ACEA Biosciences model NovoCyte 2060, AP.EMU
18/14881-2 - Multi-User Equipment approved in grant 2018/05006-0: ultramicrotome Leica UC7, AP.EMU
Associated scholarship(s):19/06591-7 - Establishment of a model for spinal root reimplantation after avulsion in C57BL/6J mice: use of fibrin polymer and dimethyl-fumarate, BP.MS
19/02714-7 - Involvement of MHC i, TLR2 and TLR4 in the synaptic plasticity and functional reovery following crushing of spinal ventral roots in mice, BP.PD
18/25845-7 - Neuroprotective and immunomodulatory effect of dimethyl-fumarate (DMF) after avulsion and reimplantation of spinal motor roots associated to cell-therapy, BP.DD
19/00833-9 - Laboratory training program - Laboratory of Nerve Regeneration, BP.TT

Abstract

Motor coordination involves specific neural processes ranging from the perception of stimuli to the achievement of the response, being dependent on delicate sensory-motor integration, which is particularly evident in the spinal cord. Spinal root injuries can lead to motor, sensibility and autonomic losses. This type of lesion constitutes a significant medical problem and usually affects the brachial plexus in consequence of high energy trauma. However, due to the possibility of generating further inflammation and neuropathic pain, surgical procedures do not prioritize the repair of the afferent component. In this context, new therapies need to be developed for dorsal root repair. A promising treatment would be the use of platelet-rich plasma (PRP). Nowadays, PRP is used in a wide range of surgical procedures, and there are several scientific studies demonstrating benefits in treating various types of injuries. Also, immunomodulation and the differential expression of MHC I, TLR2 and 4 seem to interfere in the process of synaptic plasticity following injury. Stem cell therapy has also been used to achieve neuroprotection and to stimulate axonal regrowth. In the present study, we will employ different strategies to repair spinal root injury, evaluating the expression of neurotrophic factors and pro- and anti-inflammatory cytokines. We believe that our results will serve as a basis accelerating nerve regeneration and may contribute to the future clinical use of new therapeutic approaches, fulfilling a critical gap in reparative procedures after this type of injury. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIBEIRO, PATRICIA; CASTRO, V, MATEUS; PEREZ, MATHEUS; CARTAROZZI, LUCIANA P.; SPEJO, ALINE B.; CHIAROTTO, GABRIELA B.; AUGUSTO, TAIZE M.; OLIVEIRA, ALEXANDRE L. R. Toll-like receptor 4 (TLR4) influences the glial reaction in the spinal cord and the neural response to injury following peripheral nerve crush. Brain Research Bulletin, v. 155, p. 67-80, FEB 2020. Web of Science Citations: 0.
CHIAROTTO, GABRIELA BORTOLANCA; CARTAROZZI, LUCIANA POLITTI; PEREZ, MATHEUS; BISCOLA, NATALIA PERUSSI; SPEJO, ALINE BARROSO; GUBERT, FERNANDA; FRANCA JUNIOR, MARCONDES; MENDEZ-OTERO, ROSALIA; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. Tempol improves neuroinflammation and delays motor dysfunction in a mouse model (SOD1(G93A)) of ALS. JOURNAL OF NEUROINFLAMMATION, v. 16, n. 1 NOV 14 2019. Web of Science Citations: 0.
DE CASTRO, MATEUS VIDIGAL; RAMOS DA SILVA, MONIZE VALERIA; CHIAROTTO, GABRIELA BORTOLANCA; VOLPE, BRUNO BOSH; SANTANA, MARIA HELENA; MALHEIROS LUZO, ANGELA CRISTINA; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. Reflex arc recovery after spinal cord dorsal root repair with platelet rich plasma (PRP). Brain Research Bulletin, v. 152, p. 212-224, OCT 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.