Advanced search
Start date
Betweenand

Comparative biochemical study of gill (Na+, K +)- ATPase of Macrobrachium amazonicum (Heller, 1862) inhabitant of coastal and continental regions

Grant number: 16/25336-0
Support type:Regular Research Grants
Duration: April 01, 2017 - August 31, 2019
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Francisco de Assis Leone
Grantee:Francisco de Assis Leone
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers: Carlos Frederico Leite Fontes ; Cristiana Ramalho Maciel ; Daniela Pereira Garçon ; Eduardo Alves de Almeida ; Fernando Luis Medina Mantelatto ; John Campbell McNamara ; Karina Ribeiro ; Michelle Pinheiro Vetorelli ; Wagner Cotroni Valenti

Abstract

This project is a continuation of the research line that has been supported by FAPESP (2013/22605-1; 2013/00976-8 ; 2013/24252-9 ; 2010/17534-0; 2010/16115-3; 2010/06395-9; 2006/56435-1; 2006/56759-1; 2003/01233-7. The objective is the systematic study of the structural and kinetic properties of the (Na,K)-ATPase in the gill tissue of different species of crabs and ATPase shrimps, aiming to establish a model for the adaptation of crustaceans to different salinity environments. The maintenance of osmolality of the hemolymph of crustaceans is attributed to the (Na,K)-ATPase and this adaptation mechanism suggests that the responses of crustaceans involve a rapid activation of ion transport that should be primary to any other physiological response of this animal.The objective of the present project is to evaluate comparatively the kinetic and molecular characteristics of the gill (Na,K)-ATPase of M. amazonicum populations inhabiting both coastal and continental areas, in order to access the elucidation of the physiological mechanisms of geographically separated entities and trying to clarify whether different populations of M. amazonicum found in different parts of the country are originated from a single species or they represent indeed different species. To achieve the objectives the following steps will be are performed : preparation of membrane-rich gill (Na,K)-ATPase from shrimps collected in different habitats; electrophoresis and Western blotting; determination of osmolality and ion concentrations in the hemolymph; mRNA expression of alpha subunit of (Na,K)-ATPase and V(H)-ATPase; FXYD2 effect on (Na,K)-ATPase activity. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LUCENA, MALSON N.; GARCON, DANIELA P.; FONTES, CARLOS F. L.; FABRI, LEONARDO M.; MORAES, CINTYA M.; MCNAMARA, JOHN C.; LEONE, FRANCISCO A. Dopamine binding directly up-regulates (Na+, K+)-ATPase activity in the gills of the freshwater shrimp Macrobrachium amazonicum. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 233, p. 39-47, JUL 2019. Web of Science Citations: 0.
FABRI, LEONARDO M.; LUCENA, MALSON N.; GARCON, DANIELA P.; MORAES, CINTYA M.; MCNAMARA, JOHN C.; LEONE, FRANCISCO A. Kinetic characterization of the gill (Na+, K+)-ATPase in a hololimnetic population of the diadromous Amazon River shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 227, p. 64-74, JAN 2019. Web of Science Citations: 1.
FALEIROS, ROGERIO O.; GARCON, DANIELA P.; LUCENA, MALSON N.; MCNAMARA, JOHN C.; LEONE, FRANCISCO A. Short- and long-term salinity challenge, osmoregulatory ability, and (Na+, K+)-ATPase kinetics and alpha-subunit mRNA expression in the gills of the thinstripe hermit crab Clibanarius symmetricus (Anomura, Diogenidae). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 225, p. 16-25, NOV 2018. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.