TISSUE RESPONSE IN A CRITICAL SIZED DEFECT OF RATS CALVARIA TREATED WITH A NIOBIUM DOPED BIOACTIVE GLASS: IN VITRO AND IN VIVO STUDY.

• Grant number: 16/24372-2
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2017
• End date: March 31, 2019
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Jose Angelo Camilli
• Grantee: Jose Angelo Camilli
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Celso Aparecido Bertran ; João Henrique Lopes

Abstract

Introduction: The introduction of niobium (Nb2O5) into bioactive glasses is been shown to improve their mechanical properties improving the material's bioactivity which is related to a higher capacity of stimulating regeneration of fractures. The mechanisms by which niobium doped glasses act over living cells are still unknown as well as its cytotoxic and mutagenic potential. Objective: The aim of this study is to analyse local and systemic responses of niobium doped bioactive glasses implanted in rats, as well as, the effects of exposing Osteoblasts and Mesenchymal stem cells to the glasses' dissolution products. Methods: It will be carried out in vivo and in vitro experiments. The in vivo assay will consist of creating a critical sized circular defect on rats calvaria which will be filled with different compositions of bioactive glasses in the form of powder. The osteointegration will be assessed by histomorphometry. Signalling molecules related to different processes that occur during fracture regeneration will be quantified in the blood plasma and in the injured bone by means of ELISA kits. Furthermore, systemic cytotoxicity will be verified by histopatological examination of HE stained slides of liver, kidneys and heart. The mutagenic potential of the implants will be assessed by micronucleus test. In the in vitro assay it will be investigated the differentiation, maturation and matrix mineralization of osteoblasts and mesenchymal stem cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, UCAS P. L.; LOPES, JOAO H.; FERREIRA, V, FILIPE; MARTIN, RICHARD A.; BERTRAN, CELSO A.; CAMILLI, JOSE A.. Evaluation of effectiveness of 45S5 bioglass doped with niobium for repairing critical-sized bone defect in in vitro and in vivo models. Journal of Biomedical Materials Research Part A, v. 108, n. 3, NOV 2019. (10/05394-9, 16/24372-2)