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Tissue response in a critical sized defect of rats calvaria treated with a niobium doped bioactive glass: in vitro and in vivo study

Grant number: 16/24372-2
Support type:Regular Research Grants
Duration: April 01, 2017 - March 31, 2019
Field of knowledge:Biological Sciences - Morphology
Principal Investigator:Jose Angelo Camilli
Grantee:Jose Angelo Camilli
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers:Celso Aparecido Bertran ; João Henrique Lopes


Introduction: The introduction of niobium (Nb2O5) into bioactive glasses is been shown to improve their mechanical properties improving the material's bioactivity which is related to a higher capacity of stimulating regeneration of fractures. The mechanisms by which niobium doped glasses act over living cells are still unknown as well as its cytotoxic and mutagenic potential. Objective: The aim of this study is to analyse local and systemic responses of niobium doped bioactive glasses implanted in rats, as well as, the effects of exposing Osteoblasts and Mesenchymal stem cells to the glasses' dissolution products. Methods: It will be carried out in vivo and in vitro experiments. The in vivo assay will consist of creating a critical sized circular defect on rats calvaria which will be filled with different compositions of bioactive glasses in the form of powder. The osteointegration will be assessed by histomorphometry. Signalling molecules related to different processes that occur during fracture regeneration will be quantified in the blood plasma and in the injured bone by means of ELISA kits. Furthermore, systemic cytotoxicity will be verified by histopatological examination of HE stained slides of liver, kidneys and heart. The mutagenic potential of the implants will be assessed by micronucleus test. In the in vitro assay it will be investigated the differentiation, maturation and matrix mineralization of osteoblasts and mesenchymal stem cells. (AU)