Investigation of posttranslational histone modifications and co-regulators of expression in the Schistosoma mansoni stages

• Grant number: 17/07364-9
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2017
• End date: January 31, 2020
• Field of knowledge: Biological Sciences - Parasitology - Helminthology of Parasites
• Principal Investigator: Fernanda Janku Cabral
• Grantee: Fernanda Janku Cabral
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Christoph Grunau ; Emanuel Carrilho ; Leo Kei Iwai ; Silmara Marques Allegretti

Abstract

Schistosomiasis is caused by the parasite Schistosoma mansoni and is prevalent in tropical and subtropical areas of the world. It is estimated that it affects 200 million people and more than 1,53 million people are disabled by the disease. S. mansoni presents a complex life cycle with an intriguing development and although several efforts have been made to study genes, transcripts and epigenome of the parasite, little is known about how gene expression is regulated. In the attempt to understand the control of the gene expression in the life cycle of the parasite, the aim of this project is to investigate the importance of posttranslational histone tail modifications and whether these epigenetic marks are dynamically acquired and lost during stages as well as the putative transcriptional co-regulators that contribute to the gene expression control of the parasite. Specifically, it is proposed to investigate: 1) the dynamics of chromatin modification using mass spectrometry; 2) elaboration of a comparative map between histone PTMs and gene expression of annotated modifying enzymes in the stages; 3) putative transcriptional co-regulators such as HP1 that may play a role in the regulation of silencing in cercariae. Finally, with this project we can advance in knowledge on the regulation of different parasite stages gene expression and may find new strategies to treat this disease. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA TRINDADE, NATALIA SILVA; DE SOUSA CARDOSO, THAIS CUNHA; COSTA, GIULLIANA GALDINI; CRUVINEL, GABRIELA FREITAS; MASSON, ANA PAULA; LANFREDI, GUILHERME PAUPERIO; ALLEGRETTI, SILMARA MARQUES; FACA, VITOR; GOMES, MATHEUS DE SOUZA; MORAIS, ENYARA REZENDE; et al. chistosoma mansoni Heterochromatin Protein 1 (HP1) nuclear interactome in cercaria. JOURNAL OF PROTEOMICS, v. 239, MAY 15 2021. (17/07364-9)

MENDES, TIAGO M. F.; CARRILHO, EMANUEL; GALINARO, CARLOS A.; CABRAL, FERNANDA J.; ALLEGRETTI, SILMARA M.. Biomphalaria glabrata infected with Angiostrongylus cantonensis: Proteomic changes in the snail host. Acta Tropica, v. 212, DEC 2020. (17/07364-9, 16/07137-0, 09/54040-8)

DA TRINDADE, NATALIA SILVA; DE SOUSA CARDOSO, THAIS CUNHA; COSTA, GIULLIANA GALDINI; CRUVINEL, GABRIELA FREITAS; MASSON, ANA PAULA; LANFREDI, GUILHERME PAUPERIO; ALLEGRETTI, SILMARA MARQUES; FACA, VITOR; GOMES, MATHEUS DE SOUZA; MORAIS, ENYARA REZENDE; et al. Schistosoma mansoni Heterochromatin Protein 1 (HP1) nuclear interactome in cercariae. JOURNAL OF PROTEOMICS, v. 239, p. 12-pg., 2021-03-13. (17/07364-9)

LOBO-SILVA, JESSICA; CABRAL, FERNANDA J.; AMARAL, MURILO S.; MIYASATO, PATRICIA A.; DE FREITAS, RAFAELA PAULA; PEREIRA, ADRIANA S. A.; KHOURI, I, MARIANA; BARBOSA, MAYRA M. F.; RAMOS, PABLO I. P.; LEITE, LUCIANA C. C.; et al. The antischistosomal potential of GSK-J4, an H3K27 demethylase inhibitor: insights from molecular modeling, transcriptomics and in vitro assays. PARASITES & VECTORS, v. 13, n. 1, MAR 17 2020. (15/06366-2, 17/07364-9)

FERNANDES MENDES, TIAGO MANUEL; CARRILHO, EMANUEL; FONSECA SIEUVE AFONSO, ANA JULIA PINTO; GALINARO, CARLOS ALEXANDRE; CABRAL, FERNANDA JANKU; ALLEGRETTI, SILMARA MARQUES. Proteomic, metabolic and immunological changes in Biomphalaria glabrata infected with Schistosoma mansoni. International Journal for Parasitology, v. 49, n. 13-14, p. 1049-1060, DEC 2019. (09/54040-8, 16/07137-0, 17/07364-9)

DO PATROCINIO, ANDRESSA BARBAN; CABRAL, FERNANDA JANKU; BRANDAO BITENCOURT, ANDRE LUIZ; BRIGATO, OLINDA MARA; MAGALHAES, LIZANDRA GUIDI; DE LIMA PAULA, LUCAS ANTONIO; FRANCO, LARISSA; GUERRA-SA, RENATA; RODRIGUES, VANDERLEI. Inhibition of 19S proteasome deubiquitinating activity in Schistosoma mansoni affects viability, oviposition, and structural changes. Parasitology Research, v. 119, n. 7, MAY 2020. (16/06769-2, 17/07364-9)

DO PATROCINIO, ANDRESSA BARBAN; CABRAL, FERNANDA JANKU; DE PAIVA, THALES HENRIQUE; MAGALHAES, LIZANDRA GUIDI; DE LIMA PAULA, LUCAS ANTONIO; BRIGATO, OLINDA MARA; GUERRA-SA, RENATA; RODRIGUES, VANDERLEI. Deubiquitinating enzymes as possible drug targets for schistosomiasis. Acta Tropica, v. 217, MAY 2021. (16/06769-2, 17/07364-9)