Cardiac remodeling in rats with right heart dysfunction subjected to preventive physical training: morphofunctional and molecular study.

• Grant number: 16/11344-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2017
• End date: February 29, 2020
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Francis Lopes Pacagnelli
• Grantee: Francis Lopes Pacagnelli
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil
• Associated researchers: Antonio Carlos Cicogna ; Katashi Okoshi ; Rejane Batista Brinholi Victorino da Silva ; Yudney Pereira da Motta
• Associated scholarship(s): 18/24317-7 - Analysis of the molecular pathways involving the collagen in the right ventricle of rats with pulmonary hypertension submitted to physical training, BP.TT

Abstract

Right heart failure is an evolution of ventricular dysfunction and is related to functional limitations and high rates of morbidity and mortality. Exercise, in a preventative manner, acting on the stage of cardiac dysfunction that precedes HF, could be beneficial in cardiac disorders and has not been widely studied.Objective: to test the hypothesis that preventive physical training in animals with right ventricular dysfunction will promote attenuation of cardiac alterations through morphofunctional and molecular methods. Methods: 32 male Wistar rats will be used, divided into 4 groups (n = 8): sedentary control; training control; sedentary monocrotaline; training monocrotaline.The preventive training protocol will be performed on a treadmill for 13 weeks, 5 times/week, with a 2 week adaptation period and gradual increases in speed/time. In the third, fourth and fifth weeks, the training will be performed at 0.9km/h-60min, in the sixth, seventh and eighth weeks at 1km/h-60min and in the ninth and tenth weeks at 1.1km/h-60min.Next, the monocrotaline(60mg/Kg) will be administered to induce pulmonary arterial hypertension and the lactate threshold analyzed to determine the training speeds for the following 3 weeks. After identification of ventricular dysfunction through echocardiography, the animals will be euthanized. Cardiac function will be evaluated through an echocardiogram, right ventricular hypertrophy through histomorphometric analysis and the mechanisms of cardiac dysfunction using gene expression (RT-qPCR) and protein analysis (Western Blotting).Normality of the data will be analyzed using the Shapiro-Wilk test and data comparisons using the ANOVA with Tukey's or Dunn's post test (p <5%). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PACAGNELLI, F. L.; SABELA, ALMEIDA A. K.; OKOSHI, K.; MARIANO, T. B.; CAMPOS, D. H.; CARVALHO, R. F.; CICOGN, A. C.; VANDERLEI, L. C.. Preventive aerobic training exerts a cardioprotective effect on rats treated with monocrotaline (vol 97, pg 238, 2016). International Journal of Experimental Pathology, v. 99, n. 3, p. 1-pg., 2018-06-01. (16/11344-0)

GASPAR, MATHEUS Z.; YABUNAKA, ANA C.; SILVA-CARVALHO, ROSIMEIRE; NASCIMENTO, CHARLES U.; BRINHOLI, REJANE B.; SILVA, ELISANGELA O.; GEREZ, JULIANA R.; SILVA JR, NELSON J.; TORRES-BONILLA, KRISTIAN A.; HYSLOP, STEPHEN; et al. Cardiac Effects of Micrurus corallinus and Micrurus dumerilii carinicauda (Elapidae) Venoms and Neutralization by Brazilian Coralsnake Antivenom and Varespladib. Cardiovascular Toxicology, v. 23, n. 3-4, p. 15-pg., 2023-02-22. (16/11344-0, 20/14191-6, 20/04287-6)