Characterization and study of Enzymes to biomass degradation.

• Grant number: 17/25705-8
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2018
• End date: February 29, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Felipe Santiago Chambergo Alcalde
• Grantee: Felipe Santiago Chambergo Alcalde
• Host Institution: Escola de Artes, Ciências e Humanidades (EACH). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

During the enzymatic hydrolysis of lignocellulosic biomass mixtures of enzymes are used for soluble fermentable sugars. The main enzymes in the mixture are: cellobiohydrolase, endoglucanases, b-glucosidase, a-xylosidases, a-arabino- furanosidases, pectinases, feruloyl esterase, oxidoreductase and other accessory enzymes. Recent research has demonstrated the participation and importance of several main and auxiliary / accessory enzymes in the preparation of the cocktail of enzymes involved in the lignocellulose degradation process. The present proposal aims at the study and characterization of enzymes (±-L-arabinofuranosidase, xylanase, feruloyl esterase e rhamnogalacturonan acetyl esterase) with potential use in the enzymatic cocktail to obtain reducing of sugarcane. The identification of mixtures of enzymes with potential use in lignocellulose hydrolysis will allow the efficient enzymatic treatment and obtaining a higher level of sugars from the biomass. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, GABRIEL S.; ADRIANI, PATRICIA P.; RIBEIRO, JOAO AUGUSTO; MORISSEAU, CHRISTOPHE; HAMMOCK, BRUCE D.; DIAS, MARCIO VINICIUS B.; CHAMBERGO, FELIPE S.. The molecular structure of an epoxide hydrolase from Trichoderma reesei in complex with urea or amide-based inhibitors. International Journal of Biological Macromolecules, v. 129, p. 653-658, MAY 15 2019. (13/15906-5, 16/12859-4, 15/03329-9, 15/09188-8, 18/00351-1, 14/24107-1, 17/25705-8)

ADRIANI, PATRICIA P.; DE PAIVA, FERNANDA C. R.; DE OLIVEIRA, GABRIEL S.; LEITE, AMANDA C.; SANCHES, ADRIANA S.; LOPES, ADRIANA RIOS; DIAS, MARCIO V. B.; CHAMBERGO, FELIPE S.. Structural and functional characterization of the glutathione peroxidase-like thioredoxin peroxidase from the fungus Trichoderma reesei. International Journal of Biological Macromolecules, v. 167, p. 93-100, JAN 15 2021. (18/00351-1, 17/25705-8, 19/05227-0, 14/24107-1)

DE OLIVEIRA, GABRIEL S.; ADRIANI, PATRICIA P.; WU, HAO; MORISSEAU, CHRISTOPHE; HAMMOCK, BRUCE D.; CHAMBERGO, FELIPE S.. Substrate and inhibitor selectivity, and biological activity of an epoxide hydrolase from Trichoderma reesei. MOLECULAR BIOLOGY REPORTS, v. 46, n. 1, p. 371-379, FEB 2019. (16/12859-4, 15/03329-9, 14/24107-1, 17/25705-8)

DE MELO, VANDIERLY SAMPAIO; GOMES, BRISA MOREIRA; CHAMBERGO, FELIPE SANTIAGO. Biochemical characterization of a xylose-tolerant GH43 β-xylosidase from Geobacillus thermodenitrificans. Carbohydrate Research, v. 532, p. 8-pg., 2023-10-01. (17/25705-8)