Sustainable citrus waste biorefining as a source of value added bioproducts

• Grant number: 17/50303-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2018
• End date: March 31, 2020
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Agreement: University of Manchester
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Valéria de Carvalho Santos Ebinuma
• Grantee: Valéria de Carvalho Santos Ebinuma
• Principal researcher abroad: James Winterburn
• Institution abroad: University of Manchester , England
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• City of the host institution: Araraquara

Abstract

The main challenge which limits the wider uptake of biobased chemicals, produced by biological transfonmation via microbial fermentation, as altematives to those derived from petroleum is the cost of production. The majority of the direct costs of such bioprocesses are accounted for by the substrate used. This presents an opportunity for the development of biorefineries engineered to take sugar (carbon) and nutrient rich wastes and by-products and tum them into value added biomolecules. One such inefficiently used agro-industrial waste is citrus processing waste, predominately orange juicing waste. In a typical orange juicing process around 50% of the processed mass of oranges leaves as waste, which is usually disposed of by incineration to recover some energy. As a sugar rich waste stream orange peels can be used as a low cost substrate to obtain biomolecules. The key aim of the current joint proposal is to develop a technically feasible citrus waste biorefinery, by engineering a bioprocess to obtain a sugar rich fermentation media for the production of biocolorants using Ta/aromyces amestolkiae and biopolymers using Cupriavidus necator H16. Alongside the development of the bioprocess as a whole, the environmental and economic irnpacts, of such an orange juicing waste biorefinery will be assessed on a life cycle basis. The proposed work will support the current Young Investigators FAPESP project, n. 2014/01580-3, entitled "Biotechnological process for the development of new natural colorants from microorganisms for industrial application", which is coordinated by Dr. Valéria C. S. Ebinuma. Furthermore, this collaboration will underpin the development of a broader, high quality biorefining research network to bring together Brazilian (where sugarcane to ethanol biorefining is well established) and UK biorefining expertise, promoting the valorisation of agricultural waste streams, including citrus by-products, through transformation into value-added bioproducts. The fonmation of this network will enable two-way knowledge exchange and provide the foundation for future funded joint-projects. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUSSAGY, CASSAMO USSEMANE; WINTERBURN, JAMES; SANTOS-EBINUMA, VALERIA CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Production and extraction of carotenoids produced by microorganisms. Applied Microbiology and Biotechnology, v. 103, n. 3, p. 1095-1114, FEB 2019. (17/50303-0, 14/16424-7, 14/01580-3, 14/19793-3)

MUSSAGY, CASSAMO U.; SANTOS-EBINUMA, VALERIA C.; GONZALEZ-MIQUEL, MARIA; COUTINHO, JOAO A. P.; PEREIRA, JORGE F. B.. Protic Ionic Liquids as Cell-Disrupting Agents for the Recovery of Intracellular Carotenoids from Yeast Rhodotorula glutinis CCT-2186. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 7, n. 19, p. 16765-16776, OCT 7 2019. (14/16424-7, 14/01580-3, 14/19793-3, 17/50303-0)

DE OLIVEIRA, FERNANDA; FERREIRA, LAURA CARMONA; BAPTISTA NETO, ALVARO; SIMAS TEIXEIRA, MARIA FRANCISCA; SANTOS EBINUMA, VALERIA DE CARVALHO. Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor. Biochemical Engineering Journal, v. 161, SEP 15 2020. (17/50303-0, 14/01580-3, 19/18076-0)

MUSSAGY, CASSAMO U.; PEREIRA, JORGE F. B.; DUFOSSE, LAURENT; RAGHAVAN, VIJAYA; SANTOS-EBINUMA, VALERIA C.; PESSOA JR, ADALBERTO. Advances and trends in biotechnological production of natural astaxanthin by Phaffia rhodozyma yeast. CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, AUG 2021. (17/50303-0, 15/50058-0, 14/19793-3, 14/16424-7, 18/06908-8, 20/08655-0)

MUSSAGY, CASSAMO USSEMANE; CARIZIA GUIMARAES, ANA ALICE; FERREIRA ROCHA, LARA VICENTE; WINTERBURN, JAMES; SANTOS-EBINUMA, VALERIA DE CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Improvement of carotenoids production from Rhodotorula glutinis CCT-2186. Biochemical Engineering Journal, v. 165, JAN 15 2021. (17/50303-0, 14/16424-7, 14/01580-3, 14/19793-3, 19/09618-3)

VIEIRA, SUELLEN ROCHA; ALVES DA SILVA, JANIA BETANIA; DRUZIAN, JANICE IZABEL; ASSIS, DENILSON DE JESUS; MUSSAGE, CASSAMO USSEMANE; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA CARVALHO; FRANCA LEMOS, PAULO VITOR; CORREIA, PAULO ROMANO; FERREIRA, EDERLAN DE SOUZA; et al. Cellulose Nanoparticles Prepared by Ionic Liquid-Assisted Method Improve the Properties of Bionanocomposite Films. JOURNAL OF POLYMERS AND THE ENVIRONMENT, v. N/A, p. 12-pg., 2022-03-20. (17/50303-0, 15/50058-0)

MUSSAGY, CASSAMO U.; SANTOS-EBINUMA, VALERIA C.; KURNIA, KIKI A.; DIAS, V, ANA C. R.; CARVALHO, PEDRO; COUTINHO, JOAO A. P.; PEREIRA, JORGE F. B.. Integrative platform for the selective recovery of intracellular carotenoids and lipids from Rhodotorula glutinis CCT-2186 yeast using mixtures of bio-based solvents. GREEN CHEMISTRY, v. 22, n. 23, p. 8478-8494, DEC 7 2020. (14/16424-7, 15/50058-0, 14/19793-3, 17/50303-0)