Effect of oxidative stress on maturation and culture of in vitro produced embryos in swine

• Grant number: 05/01420-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2005
• End date: April 30, 2009
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: José Antonio Visintin
• Grantee: Mariana Groke Marques
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The aim of this study will be to investigate de enficiency of controled atmosphere (5% CO2, 5% O2 and 90% N2) on oocyte maturation with defined cuture medium or supplemented with porcine folicular fluid (PFF) and also on in vitro embryo development. Oocytes will be in vitro matured (IVM) for 44 hours in two culture medium: TCM199 supplemented with PVA 0,1%, or PFF10% with controlled atmosphere or not. After IVM an oocyte sample will be analized for nuclear and citoplasmatic maturations, glutathione concentration and oxidative stress (HSC70 protein formation). The remaining oocytes will be in vitro fertilized (IVF) and cultured under controlled atmosphere system or not. To access IVF rate after 18 hours culture, polismermy will be evaluated. Embryo culture will be estimated by clivage and blastocyst rates at Day 3 and 6, respectively, by differencial staining of trophectoderm cell (TF) and inner cell mass (ICM) and by HSC70 protein formation (oxidative stress).

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARQUES, Mariana Groke. Effect of oxygen tension and media on in vitro oocyte maturation and its influence in the porcine embryonic development. 2009. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.