Solid sensors for nitric oxide detection: obtention and application in biological systems

• Grant number: 06/04408-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2007
• End date: March 31, 2008
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Carlos Frederico de Oliveira Graeff
• Grantee: Mayler Martins
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Nitric oxide (NO) is a free radical that acts in many tissues to regulate a diverse range of physiological process and has an important role in the endothelial muscular relaxation. The development of a sensor for quantitative NO detection “in vitro” or “ex-vivo” is of great importance to studies of NO generation and its physiological role mechanism in biological systems.The purpose of this work is to synthesize and characterize NO sensors obtained thought molecules encapsulation in a porous matrix. The sensors proposed is based on the encapsulation iron(II)-diethyldithiocarbamic Fe2+(DETC2) complex in a siloxane matrix by sol-gel process or in a latex. The NO detection and sensor characterization will be performed by means of electron paramagnetic resonance (EPR) and optical methods. Structural and morphological sensor properties will be study by means of Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), thermal analysis, and others. Biological studies in cellular culture will also be performed.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARTINS, Mayler. Sensores sólidos para detecção de óxido nítrico baseados em cucurbit[6]uril e em siloxano-poli(oxipropileno). 2011. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências. Bauru Bauru.