Molecular analysis of microorganisms found in deciduous teeth endodontic infections

• Grant number: 07/03577-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2007
• End date: October 31, 2010
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Mario Júlio Ávila-Campos
• Grantee: Antônio Scalco Fabris
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Few researches have been performed for determining the bacteria involved in deciduous teeth with pulpy necrosis and periapical infection. Microorganisms involved in these infections are not easily cultivated and it justify the use of molecular techniques for studying that microbiota to know better the involved agents and to take a better therapeutic orientation. One hundred deciduous teeth with caries will be analyzed with or none presence of fistula. For each clinical sample will be made a stain by Gram and Brenn & Brown methods to determine the microbial morphotypes and the PCR based in the 16S rDNA will also be used. Moreover, in the positive samples to bacteria,will be detect genes involved with the antimicrobial resistance: ermA (erythromycin and lincomicin), tetQ and tetW (tetracycline), and cfxA ( beta lactamase production).

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FABRIS, Antônio Scalco. Bacteriological analysis of pulp infection in deciduous teeth.. 2011. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.