Bacteriological analysis of pulp infection in deciduous teeth.

Author(s)

Antonio Scalco Fabris

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-05-16

Examining board members

Mario Julio Avila Campos; Mariana Minatel Braga Fraga; Jose Carlos Pettorossi Imparato; Rosana de Souza Pereira; Maria Regina Lorenzetti Simionato

• Advisor: Mario Julio Avila Campos

Abstract

In this study, deciduous teeth with deep caries from 110 children, with 103 pulp necrosis and 7 gingival fistula samples were evaluated. Bacterial morphotypes were visualized by Gram staining and Brown-Brenn. DNA were obtained and used in bacterial detection by using PCR. The predominance of Gram-positive cocci (81.8%) and Gram-negative coccobacilli (49.1%) were observed. In 88 pulp samples a high frequency of microrganisms were observed: Enterococcus spp. (50%), P. gingivalis (49%), F. nucleatum (25%) and P. nigrescens (11.4%). In fistulas were detected: P. gingivalis (43%), Enterococcus spp. (28.6%), F. nucleatum (14.3%), P. nigrescens (14.3%), and Dialister pneumosintes (14.3%). Our results, suggest that the involved microbiota in pulp infections of deciduous teeth are qualitatively similar than those permanent teeth. However, a predominance of Enterococcus spp. and P. gingivalis was observed, and it must be considered in the endodontic treatment in children with primary dentition. (AU)

• FAPESP's process: 07/03577-6 - Molecular analysis of microorganisms found in deciduous teeth endodontic infections
• Grantee: Antônio Scalco Fabris
• Support Opportunities: Scholarships in Brazil - Doctorate