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Quantitative study of infection Babesia bovis and Babesia bigemina in different genetic groups of beef cattle

Grant number: 09/12961-0
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2010
Effective date (End): July 31, 2011
Field of knowledge:Agronomical Sciences - Animal Husbandry
Principal Investigator:Henrique Nunes de Oliveira
Grantee:Talita Barban Bilhassi
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The technique of qPCR reaction (quantitative polymerase chain reaction) will be used to evaluate the intensity of infection with Babesia bovis and Babesia bigemina in cattle. Will be used groups of 25 calves and 25 beef cows of three different genetic groups (Nelore, ½ + ½ Nelore Bos taurus and pure Bos taurus). Each animal will be taking blood samples from the jugular vein and peripheral vessels, for extraction of DNA, serology and making blood smears. The quantitative PCR reactions are prepared with DNA extracted from blood collected with EDTA. The verification of the occurrence of parasitemia will be made through direct counting of parasites in blood smears stained with Giemsa, prepared from peripheral blood. The results will be analyzed to find possible differences between the genetic groups studied concerning the intensity of infection and immune response babesias aiming factors related to clinical causes of variation in resistance to endoparasites and provide subsidies for the identification of animals resistant the establishment of more effective ways to control these parasites.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BILHASSI, TALITA B.; OLIVEIRA, HENRIQUE N.; IBELLI, ADRIANA M. G.; GIGLIOTI, RODRIGO; REGITANO, LUCIANA C. A.; OLIVEIRA-SEQUEIRA, TERESA C. G.; BRESSANI, FLAVIA A.; MALAGO, JR., WILSON; RESENDE, FLAVIO D.; OLIVEIRA, MARCIA C. S. Quantitative study of Babesia bovis infection in beef cattle from Sao Paulo state, Brazil. TICKS AND TICK-BORNE DISEASES, v. 5, n. 3, p. 234-238, 2014. Web of Science Citations: 9.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BILHASSI, Talita Barban. Estudo quantitativo da infecção por Babesia bovis e Babesia bigemina em diferentes grupos genéticos de bovinos de corte. 2011. 75 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.