The technique of qPCR reaction (quantitative polymerase chain reaction) will be used to evaluate the intensity of infection with Babesia bovis and Babesia bigemina in cattle. Will be used groups of 25 calves and 25 beef cows of three different genetic groups (Nelore, ½ + ½ Nelore Bos taurus and pure Bos taurus). Each animal will be taking blood samples from the jugular vein and peripheral vessels, for extraction of DNA, serology and making blood smears. The quantitative PCR reactions are prepared with DNA extracted from blood collected with EDTA. The verification of the occurrence of parasitemia will be made through direct counting of parasites in blood smears stained with Giemsa, prepared from peripheral blood. The results will be analyzed to find possible differences between the genetic groups studied concerning the intensity of infection and immune response babesias aiming factors related to clinical causes of variation in resistance to endoparasites and provide subsidies for the identification of animals resistant the establishment of more effective ways to control these parasites.
News published in Agência FAPESP Newsletter about the scholarship: