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Comparison between top down and bottom up processing to the scaling up of the production of functional liposomes for genetic vaccination against tuberculosis

Grant number: 08/07609-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2009
Effective date (End): May 31, 2010
Field of knowledge:Engineering - Chemical Engineering - Chemical Process Industries
Principal Investigator:Maria Helena Andrade Santana
Grantee:Julia Exaltação Trevisan
Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


The present project aims to study scalable processes for the production of cationic liposomes to genic vaccination against tuberculosis. The scalable processes studied are top-down and bottom-up types. The first is based on the reduction of size of pre-formed liposomes, to the nanometric scale, through the application of impact and shear forces. Otherwise, the bottom-up approach uses the principles of microfluidics, associated to the interaction and diffusion in the interface of immiscible liquids, in order to form liposoes from the organization of molecules in the desirable size and polidispersity. The iposomes will be composed by the functional lipids: egg phosphatidyl choline (EPC),1,2-dioleoyl-sn-glicero-3-fosfoethanolamine (DOPE) e 1,2-dioleoyl-3-trimetilamonium-propane (DOTAP). Ethanol will be thesolent used. Previous studies of our group showed that that liposomes prepared in laboratorial scale by the dehidration-hidration method, when complexing DNAhsp65 on their surface, presented profilatic efficiency in mouse, when administered by intranasal route in a single dose, with a lower dosage, (25 µg ), compared to naked DNA or the same liposomes administered by intramuscular route. These results generated the submission of 01 patent and 01 addition certificate, 03 scientific articles and the approval of a PIPE FAPESP project, fase II (process n. 2007/01396-4) to the study of the scaling up of cationic functional liposomes to tuberculosis vaccination, to which this project is vinculated. The results obtained contribute to the development of processes for production of nanometric particles and to the production of the genic vaccine DNAhsp65 against tuberculosis.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TREVISAN, Julia Exaltação. Comparison between top-down and bottom-up processes for the production of functional liposomes applied on the genic vaccination agaist tuberculosis. 2010. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química Campinas, SP.

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