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Snake venomics and antivenomics of Crotalus durissus subspecies from Brazil and expression of a protease of biotechnological interest cloned from C. d. collilineatus venom gland

Grant number: 08/11688-5
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2009
Effective date (End): January 31, 2013
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Eliane Candiani Arantes Braga
Grantee:Johara Boldrini França
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The nerve growth factors (NGFs) belong to the family of neurotrophins and are responsible for modulating the development, maintaining and survival of neurons from the central and peripheral nervous system. Beyond these effects, NGFs present pleiotropic activity by activating a diversity of metabolic pathways, involved or not to the nervous system. Alterations on production transport and sensibility related to these proteins in organisms may result in the development of a variety of diseases, mainly the neurodegenerative ones. Thus, there are diverse therapeutic applications been attributed to these factors. This study has as goal express in Pichia pastoris a NGF identified from the partial cDNA library of Crotalus durissus colillineatus venom gland and the mapping of the sites responsible for the neurotrophic activity of this factor by the site-directed mutagenesis method. The snake venoms consist in important sources of growth factors. Considering this fact, the neurotrophins from this origin represent interesting objects of study, with the aim of elucidate aspects related to the activities and applications of these factors. However, there are few works on the literature related to NGF from snake venoms, what emphasize the importance of this study, which will provide relevant informations about the mechanism of action of these proteins. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA-SILVA-FREITAS, DEBORA; BOLDRINI-FRANCA, JOHARA; ARANTES, ELIANE CANDIANI. PEGylation: A Successful Approach to Improve the Biopharmaceutical Potential of Snake Venom Thrombin-Like Serine Protease. PROTEIN AND PEPTIDE LETTERS, v. 22, n. 12, p. 1133-1139, . (08/11688-5, 11/23236-4)
BOLDRINI-FRANCA, JOHARA; CORREA-NETTO, CARLOS; SILVA, MARLIETE M. S.; RODRIGUES, RENATA S.; DE LA TORRE, PILAR; PEREZ, ALICIA; SOARES, ANDREIMAR M.; ZINGALI, RUSSOLINA B.; NOGUEIRA, ROMILDO A.; RODRIGUES, VERIDIANA M.; et al. Snake venomics and antivenomics of Crotalus durissus subspecies from Brazil: Assessment of geographic variation and its implication on snakebite management. JOURNAL OF PROTEOMICS, v. 73, n. 9, p. 1758-1776, . (09/09698-5, 08/11688-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FRANÇA, Johara Boldrini. Expression of a protease of biotechnological interest cloned from C. d. collilineatus venom gland. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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