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Analysis of Antimicrobial Activity Mechanisms of Violacein against Staphylococcus aureus.

Grant number: 09/02446-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2009
Effective date (End): February 28, 2013
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Marcelo Brocchi
Grantee:Bruna de Araujo Lima
Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


This project aims to study the biological activity of violacein on different strains of Staphylococcus aureus, an important pathogen for humans and other animals through techniques of proteomics, functional genomics, analysis of antimicrobial activity (in vitro and in vivo). Previous results have shown that this compound showed bactericidal effect on strains of S. aureus, including strains of S. aureus methicillin resistant (MRSA) and S. aureus with intermediate resistance to vancomycin (VISA) in vivo model. The in vitro activity of violacein on S. aureus will be evaluated using techniques of MIC and MBC, time kill curves, protein analysis in the presence and absence of violacein (SDS-PAGE, two-dimensional electrophoresis and mass spectrometry) analysis of cellular ultrastructure by transmission electron microscopy and differential gene expression methodologies using high-throughput sequencing. The results are confirmed by real time PCR. Still, the in vivo anti-staphylococcal activity will be evaluated in an animal model.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HOLTZ, RAPHAEL D.; LIMA, BRUNA A.; SOUZA FILHO, ANTONIO G.; BROCCHI, MARCELO; ALVES, OSWALDO L.. Nanostructured silver vanadate as a promising antibacterial additive to water-based paints. Nanomedicine-Nanotechnology Biology and Medicine, v. 8, n. 6, p. 935-940, . (09/02446-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LIMA, Bruna de Araujo. Analysis of antimicrobial activity mechanisms of violacein against Staphylococcus aureus. 2013. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

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