Caracterization of death pathway, induced by metilecgonidine, product by cocaine pirolise.

• Grant number: 09/11149-0
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2010
• Effective date (End): February 29, 2012
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Tania Marcourakis
• Grantee: Lívia Mendonça Munhoz Dati
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The use of cocaine as crack is increasing when compared to other forms of use. This migration in the form of use is due to the speed of onset of pleasurable effects combined with ease of use. However, during the heating of the crack, is the formation of metilecgonidina (AEME), the pyrolysis product of cocaine. Thus, by smoking the crack, the user is exposed not only the substances found in cocaine, but also to compounds formed during heating of the stone, such as AEME, however, little is known about the effects of this compound. Previous study from our group showed that AEME has greater power in causing neurodegeneration in primary cultures of hippocampus than the cocaine and the association between the two substances. Several mechanisms leading to cell death, among them stands out to apoptosis, the necrosis, the excitotoxicity and oxidative stress. Our study suggests, through indirect methods, that cocaine triggers the process of neuronal death both by necrosis or by apoptosis, while only AEME trigger apoptosis. Accordingly, this study aims to investigate the means by which AEME, cocaine and association AEME / cocaine leads to the process of cell death in primary culture of hippocampus of the rat fetuses. Will be evaluated for both the involvement of calcium, IP3, glutamate, caspase, Bax, Bcl2, in addition to the participation of muscarinic receptors, because like the AEME with agonists of these receptors.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TAMBORELLI GARCIA, RAPHAEL CAIO; MUNHOZ DATI, LIVIA MENDONA; FUKUDA, SUELEN; LOBO TORRES, LARISSA HELENA; MOURA, SIDNEI; DE CARVALHO, NATHALIA DELAZERI; CARRETTIERO, DANIEL CARNEIRO; CAMARINI, ROSANA; LEVADA-PIRES, ADRIANA CRISTINA; YONAMINE, MAURICIO; et al. Neurotoxicity of Anhydroecgonine Methyl Ester, a Crack Cocaine Pyrolysis Product. TOXICOLOGICAL SCIENCES, v. 128, n. 1, p. 223-234, JUL 2012. (11/02734-6, 09/11149-0)

TAMBORELLI GARCIA, RAPHAEL CAIO; MUNHOZ DATI, LIVIA MENDONCA; TORRES, LARISSA HELENA; ALENCAR DA SILVA, MARIANA AGUILERA; BERTO UDO, MARIANA SAYURI; FRANCIS ABDALLA, FERNANDO MAURICIO; DA COSTA, JOSE LUIZ; GORJAO, RENATA; AFECHE, SOLANGE CASTRO; YONAMINE, MAURICIO; et al. M-1 and M-3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product. SCIENTIFIC REPORTS, v. 5, DEC 2 2015. (11/02734-6, 09/11149-0)

TAMBORELLI GARCIA, RAPHAEL CAIO; TORRES, LARISSA LOBO; MUNHOZ DATI, LIVIA MENDONCA; DE MELO LOUREIRO, ANA PAULA; AFECHE, SOLANGE CASTRO; LOPES SANDOVAL, MARIA REGINA; MARCOURAKIS, TANIA. Anhydroecgonine methyl ester (AEME), a cocaine pyrolysis product, impairs glutathione-related enzymes response and increases lipid peroxidation in the hippocampal cell culture. TOXICOLOGY REPORTS, v. 6, p. 1223-1229, 2019. (11/02734-6, 09/11149-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DATI, Lívia Mendonça Munhoz. Neurotoxicity of anydroecgonine methyl ester, a crack cocaine pyrolysis product. 2012. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.