COMPARATIVE ANALYSIS BETWEEN ACELLULAR SWINE INTESTINAL SUBMUCOSA AND SEEDED WITH HOMOLOGOUS SMOOTH MUSCLE CELLS IN TISSUE REPAIR OF BLADDER - EXPERIMENTAL STUDY IN DOGS

• Grant number: 09/12876-2
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2010
• Effective date (End): February 29, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Cláudia Valéria Seullner Brandão
• Grantee: Victor José Vieira Rossetto
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The indications for surgical repair of the bladder includes severe trauma with extensive tissue loss, cancer, interstitial cystitis applicants, neurological and congenital genitourinary abnormalities. The reconstruction of the bladder after partial cystectomy has traditionally been performed by means of transposition of segments of autologous stomach or intestine. However, according to the literature, the performance of cystoplasty with the use of gastrointestinal segments can result in numerous complications such as infection, urolithiasis and retraction of the graft. Because of these complications and with an objective to improve the functional capacity of grafts currently employed, bioengineering-based cell therapy has become a sustainable alternative. As a result, this study aims at comparing the anatomical and functional repair of the bladder in dogs using porcine intestinal submucosa acellular and seeded with smooth muscle cells counterparts through cystometry, cystography contrast and histopathology, and assess possible complications and side effects local and systemic, inherent in each type of graft employed. Additionally, it is intended to popularize the cystometry and elucidate by histopathology, immunohistochemistry and morphometry, how is the degradation of the graft porcine intestinal submucosa as replaced by the original cells of the recipient and/or autologous cells seeded on it. Finally, the study aims to make the bioengineered tissue and cell therapy, treatment options feasible in veterinary medicine and provide insight and basis for future studies in human medicine.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROSSETTO, Victor José Vieira. Submucosa intestinal suína acelular e semeada com células musculares homólogas na reparação tecidual da bexiga de cães. 2012. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.