Evaluation of genetic polymorphism of mannose-binding lectin, the gene expression of Toll-like receptors (TLR)and cytokine profile as bio-markers of cardiovascular risk in postmenopausal women

• Grant number: 09/18256-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2010
• End date: August 31, 2013
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Eliana Aguiar Petri Nahás
• Grantee: Cláudio Lera Orsatti
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Cardiovascular disease (CVD), especially coronary artery disease (CAD), comprises the main cause of mortality in postmenopausal women. CHD is caused by the atherosclerotic process of progressive evolution, which involves a number of risk factors such as hypercholesterolemia, smoking, hypertension, diabetes, stress, inactivity and obesity, which cannot be ignored in the approach to climacteric women. Through the estimation of risk, we can suggest the establishment of priority measures for primary and secondary prevention, in an attempt to reduce the occurrence of CAD. However, the detection of early markers would allow the recognition of subclinical atherosclerotic disease with early intervention on modifiable risk factors, important in management of post-menopausal women. Atherosclerosis is a disease of the arterial wall, characterized by inflammation and remodeling that can manifest as acute vascular event, becoming clinically apparent. In CAD, conventional risk factors remain important, but individual differences in immune profile may modulate the severity of the atherosclerotic process. The significance of bio-inflammatory markers in women after menopause and the risk of atherosclerosis is an important area of study. Our hypothesis is that the cytokines profile and the expression of Toll-like receptors, correlating with the genetic polymorphism of mannose-binding lecitin (MBL) prognosis is the occurrence of CHD sub-clinical ultrasound in postmenopausal menopause. It is expected that the evaluation of early biomarkers - clinical, immuno-genetic and ultrasound - of atherosclerotic disease in women after menopause may be to identify patients at cardiovascular risk or with subclinical disease thus proposing preventive measures effective in reducing the occurrence of manifest disease leading cause of mortality in this population, affecting considerably the quality of life in menopause.Aim objective of the principal project is to assess the early bio-markers - clinical, immune-genetic and ultrasound - the atherosclerotic disease in posmenopausal women followed at the Botucatu Medical School. The especific objective will be to evaluate the role of genetic polymorphism of mannose-binding lecitin (MBL), the gene expression of Toll-like receptors (TRLs) and the profile of cytokines in modulating the risk for atherosclerosis in postmenopausal women.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PETRI NAHAS, ELIANA AGUIAR. Autophagy-related 16-like 1 gene polymorphism, risk factors for cardiovascular disease and associated carotid intima-media thickness in postmenopausal women. CLINICAL BIOCHEMISTRY, v. 61, p. 12-17, NOV 2018. (09/18256-6, 09/14884-2)

ORSATTI, CLAUDIO LERA; PETRI NAHAS, ELIANA AGUIAR; NAHAS-NETO, JORGE; ORSATTI, FABIO LERA; GIORGI, VANESSA INNOCENTI; WITKIN, STEVEN S.. Evaluation of Toll-Like Receptor 2 and 4 RNA Expression and the Cytokine Profile in Postmenopausal Women with Metabolic Syndrome. PLoS One, v. 9, n. 10, OCT 17 2014. (09/18256-6, 09/14884-2)

ORSATTI, CLAUDIO LERA; PETRI NAHAS, ELIANA AGUIAR; NAHAS-NET, JORGE; ORSATTI, FABIO LERA; LINHARES, IARA MORENO; WITKIN, STEVEN SOL. Mannose-binding lectin gene polymorphism and risk factors for cardiovascular disease in postmenopausal women. Molecular Immunology, v. 61, n. 1, p. 23-27, SEP 2014. (09/18256-6, 09/14884-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ORSATTI, Cláudio Lera. Avaliação do polimorfismo genético da lecitina ligante de manose (MBL2) e da expressão gênica dos receptores Toll-Like (TLR) como bio-marcadores do risco cardiovascular em mulheres na pós-menopausa. 2014. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu Botucatu.