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Biochemical and immunological characterization of a salivary serpin from vector Aedes aegypti

Grant number: 10/00227-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2010
Effective date (End): November 30, 2010
Field of knowledge:Biological Sciences - Immunology - Cellular Immunology
Principal Investigator:Anderson de Sá Nunes
Grantee:Thaís Boccia da Costa
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:09/09892-6 - Functional immunome of Aedes aegypti saliva, AP.JP

Abstract

Insects are responsible for the transmission of several infectious diseases that affect humans and other animals, including dengue fever, malaria, leishmaniasis, yellow fever amongst other illnesses. While these insects have their blood meal, they must be able to trespass the barriers present in the host such as vasoconstriction and blood coagulation. For that purpose, the saliva of these insects is filled with anti-hemostatic constituents able to control inflammation and supress lymphocyte proliferation. Several studies dating since the 1980s have demonstrated the vasodilation and anticoagulation properties of some constituents of Aedes aegypti saliva. The catalog of mRNAs and proteins present in the saliva (saliva) expanded the identification of components responsible for these anti-hemostatic properties. In the current project, we intend to employ of the same strategy to investigate the immunomodulatory effects of Aedes aegypti saliva. The transcript AET-7393, recently identified in one of the species sialomes, was characterized as a serpin (serine protease inhibitor). Several events in the immune system are mediated by serine proteases such as the classical and the lectin of activation of the complement system, granzymes A and B found in the granules of neutrophils and CD8+ T lymphocytes, among others. Thus, our aim is to perform the biochemical characterization of this serpin and to determine its modulatory effects on the innate and acquired immunity using different in vitro and in vivo experimental approaches. (AU)