Artificial Nucleases Derived from Beta-carboline Alkaloids. Synthesis, Phosphodiester Recognition and Catalytic Activity.

• Grant number: 10/00336-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2010
• End date: August 31, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Ronaldo Aloise Pilli
• Grantee: Rodrigo Vezula Pirovani
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

This proposal aims to prepare 3 beta-carbolines displaying one or two alkylguanidine residues in order to evaluate their non-covalent interaction with 3 phosphodiesters. The methodology to be employed in these studies are: NMR, Mass Spectrometry and Calorimetry. Once the non-covalent interactions are known, the appropriate guanidine derivatives displaying an hydroxyalkyl substituent will be chosen to determine the catalytic activity on the hydrolyses of 3 phosphodiesters, under different conditions (solvent, temperature, pH, in the presence and absence of metallic cations.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIROVANI, RODRIGO V.; BRITO, GILMAR A.; BARCELOS, ROSIMEIRE C.; PILLI, RONALDO A.. Enantioselective Total Synthesis of (+)-Lyngbyabellin M. MARINE DRUGS, v. 13, n. 6, p. 3309-3324, JUN 2015. (10/00336-0, 09/51602-5, 13/07607-8, 10/00219-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PIROVANI, Rodrigo Vezula. Synthesis of potential artificial nucleases derived from the alkaloid (+/-)-trypargine and total synthesis of Lyngbyabellin M.. 2014. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.