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Molecular characterization of rumen bacteria in steers fed different roughage:concentrate diet

Grant number: 10/02036-4
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2010
Effective date (End): February 29, 2012
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:Telma Teresinha Berchielli
Grantee:Carlos Stefenson Ribeiro Junior
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


Diet is one of the main factors affecting the profile of rumen microbial, and different roughage: concentrate can dramatically influence the microbial population of the rumen, and the molecular identification of these microorganisms is an innovative and efficient characterization of rumen microbial. The present work aims to characterize the changes in rumen microbiology using molecular biotechnology and correlated to the parameters: pH, ammonia nitrogen, volatile fatty acids, as well as assess the digestibility of diets in animals fed different ratios forage: concentrate, using as a source of forage to cane sugar. Will be used 16 crossbred steers with initial body weight of 280 kg, 24 months of age, cannulated in the rumen. These animals will be distributed in a completely randomized design with 4 treatments counting (forage: concentrate 70:30, 60:40, 40:60 and 20:80 in the diet) and four repetitions (animals) per treatment and source of forage will be cane sugar fresh parsley. The data will be analyzed in the statistical program SAS (2004).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIBEIRO JUNIOR, CARLOS S.; MESSANA, JULIANA D.; GRANJA-SALCEDO, YURY T.; CANESIN, ROBERTA C.; FIORENTINI, GIOVANI; SAN VITO, ELIAS; FURLAN, LUIZ R.; REIS, RICARDO A.; BERCHIELLI, TELMA T. Parameters of fermentation and rumen microbiota of Nellore steers fed with different proportions of concentrate in fresh sugarcane containing diets. ARCHIVES OF ANIMAL NUTRITION, v. 70, n. 5, p. 402-415, 2016. Web of Science Citations: 3.
RIBEIRO, C. S.; GRANJA-SALCEDO, Y. T.; MESSANA, J. D.; NETO, A. J.; CANESIN, R. C.; FIORENTINI, G.; ALARCON, M. F. F.; BERCHIELLI, T. T. Feeding increasing concentrate to Tifton 85 hay ratios modulated rumen fermentation and microbiota in Nellore feedlot steers. JOURNAL OF AGRICULTURAL SCIENCE, v. 153, n. 6, p. 1116-1127, AUG 2015. Web of Science Citations: 4.

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