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Nanomechanical properties and micromechanical evaluation of dentin/adhesive interface after hydrolytic degradation: nanoidentation and tridimensional finite element analyses

Grant number: 10/12926-7
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2010
Effective date (End): September 30, 2013
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Eduardo Passos Rocha
Grantee:Rodolfo Bruniera Anchieta
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated scholarship(s):12/07012-1 - Nanomechanical properties and micromechanical evaluation of dentin/adhesive interface after hydrolytic degradation: a nanoindentation and tridimensional finite element analyses, BE.EP.DR


OBJECTIVE: To evaluate the nanomechanical properties and the micromechanical behavior of the dentin/adhesive interface (d/a) of specimens submitted to the degradation, through the technique of the nanoidentation (module of elasticity) and of the finite element method, in the times, 24 hours after the restoring procedures (T0) and 12 months after storage in water (T1). MATERIAL AND METHOD: The experimental model will be it of complete randomized complete blocks, being the factors of the study the adhesive systems in 5 levels and the time of analysis in 2 levels (T0, and T1), being the answer variable the module of elasticity. 5 adhesive systems will be used (5 groups): SBMU - ScotchBond Multipurpose (it Controls), ASEP - Adper SE Plus; CSEB - CLEARFIL IF BOND; P90 - self-etch adhesive P90; OUBF - One-Up-Bond F. After the obtaining of 8 specimens of each group (40 specimens), these will be stored in solution of Hanks, being used a nanoidenter (Hysitron TI 900 TriboIndenter) for the mensuration of the elastic module of the structures of the d/a interface (hybrid layer, adhesive layer, dentin) in 2 times: T0 - the measurements will be made after 24 h of the accomplishment of the bonding procedures; and T1 - the specimens will be stored in water by 12 months, and after this period they will be made the measurement. In each structure 3 indentions will be accomplished for each time of analysis. For the micromechanical analysis by the finite element analyses, 5 models of a specimen in dentin recuperated with composed resin (CR) (41x41x82 µm) will be elaborated using the SolidWorks 2010, representing structurally the groups analyzed in the nanomechanical test, being appraised in the 2 times of analysis (T0 and T1). The dimensions and mechanical properties of each group will be considered the data accordingly obtained in the nanomechanical test. The models will be considered isotropic, homogeneous and lineally elastic. The numeric analysis will be accomplished in the finite elements program's (Abaqus, Smartech). In all the models a shipment will be accomplished (C) vertical in traction, perpendicular to the surface of CR with 0.03N intensity. RESULTS: The results of the elastic module will be processed by the software of the equipment obtaining results in the scale of Mega Pascal (MPa), done being the results submit to specific statistical tests (± =0.05). The dimensions of the structures of the interface d/a (hybrid layer, adhesive layer, dentin, tag) will they be obtained by the microscope of the equipment and done compare with data of the literature. The results of the analysis micromecânica will be appraised under the criterion of the maximum main tension (Ãmax), being obtained the individualized tension maps for each structure of the d/a interface. The descriptive statistical analysis will be adopted to compare the tension values among the groups. Specific statistical tests can be used to verify the interaction of the tension values in a same model among the 2 times of analysis (T0 and T1). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANCHIETA, R. B.; ROCHA, E. P.; ALMEIDA, E. O.; FREITAS JUNIOR, A. C.; MARTIN JUNIOR, M.; MARTINI, A. P.; ARCHANGELO, C. M.; KO, C. -C.. Influence of customized composite resin fibreglass posts on the mechanics of restored treated teeth. International Endodontic Journal, v. 45, n. 2, p. 146-155, . (10/12926-7, 08/00209-9)
ANCHIETA, RODOLFO BRUNIERA; MACHADO, LUCAS SILVEIRA; MARTINI, ANA PAULA; DOS SANTOS, PAULO HENRIQUE; GIANNINI, MARCELO; JANAL, MALVIN; TOVAR, NICK; SUNDFELD, RENATO HERMAN; ROCHA, EDUARDO PASSOS; COELHO, PAULO G.. Effect of long-term storage on nanomechanical and morphological properties of dentin-adhesive interfaces. Dental Materials, v. 31, n. 2, p. 141-153, . (12/07012-1, 10/12926-7)
ANCHIETA, RODOLFO BRUNIERA; MACHADO, LUCAS SILVEIRA; SUNDFELD, RENATO HERMAN; REIS, ANDRE FIGUEIREDO; GIANNINI, MARCELO; LUERSEN, MARCO ANTONIO; JANAL, MALVIN; ROCHA, EDUARDO PASSOS; COELHO, PAULO G.. Effect of partially demineralized dentin beneath the hybrid layer on dentin-adhesive interface micromechanics. JOURNAL OF BIOMECHANICS, v. 48, n. 4, p. 701-707, . (12/07012-1, 10/12926-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)

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