Evaluation of the effect of leukotrienes in preosteoblasts proliferation and differentiation.

• Grant number: 10/13188-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2010
• End date: November 30, 2011
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Camila Peres Buzalaf
• Grantee: Amanda Amaral Pereira
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The bone metabolism controling occurs throught interaction among local and sistemic bone cells regulators. Several studies demonstrate that differences in soluble mediators such as hormones, cytokines, lipid mediators, receptors expression and signaling transduction are important to regulate the bone metabolism and pathogenesis of bone diseases. Leukotrienes (LTs) are lipid mediators of arachidonic acid-derived by the 5-lypoxigenase via and modulate the bone remodeling by altering the osteoblast and osteoclast cells function. Among them, LTB4 increases the osteoclast number and activity. Although osteoblast cells produce LTB4 and express LTs receptors, little is known about its effects on these cells. The knowledge about the machanisms by which LTs, including LTB4 and LTD4 (that bind to BLT1/2 and cysLT12 and signal differently, respectively), modulate the osteoblast differentiation may highlight the development of terapeutic estrategies in bone disease like osteoporosis. The objective of this study is to evaluate the effect of LTB4 and LTD4 and their inhibitors and/or antagonists on preosteoblasts proliferation and differentiation.