Mapping adducts generated by endogenous and exogenous aldehydes: use as biomarkers...
| Grant number: | 10/20216-0 |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| Effective date (Start): | March 01, 2011 |
| Effective date (End): | February 29, 2012 |
| Field of knowledge: | Biological Sciences - Biochemistry |
| Principal Investigator: | Marisa Helena Gennari de Medeiros |
| Grantee: | Maite Lippel Gothe |
| Home Institution: | Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
Abstract The study of the molecular mechanisms of biomolecule damage is fundamental to the understanding of redox processes and the development of biomarkers that allow studies in complex biological systems. Sensitive biomarkers can aid in understanding the cellular mechanisms involved in redox stress and have importance in the implementation of preventive strategies in several pathologies. This project aims to synthesize and characterize adducts of glutathione adducts and trans, trans-2 ,4-decadienal (DDE), aldehyde formed during the lipid peroxidation process, as a possible biomarker of redox stress. As a model we will quantify by HPLC coupled to mass spectrometry these adducts in transgenic mice model for amyotrophic lateral sclerosis. | |