Structural analysis of silk proteins of Nephila clavipes spider web by a proteomic approach.

• Grant number: 10/19051-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2011
• End date: March 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Mario Sergio Palma
• Grantee: José Roberto Aparecido dos Santos-Pinto
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated research grant: 06/57122-7 - Searching for lead compounds for rational development of new drugs and pesticides through bioprospecting in Brazilian arthropods, AP.BTA.TEM

Abstract

Strands of spider web silks are characterized by differences in their chemical composition, structure and function. Protein filaments are forming an interesting relationship of structure-function and mechanical properties, showing a strong and fairly rigid silk with an exceptional combination of strength and extensibility. Thus, it has attracted human interest in exploration and bioprospecting applications of this material which offers great potential for biomedical applications and textil industry. The spider web silk has been extensively studied using data from the genetic engineering and recombinant DNA technology. However, little or no study using a proteomics approach has been done so we can understand the structure-function relationship and interaction between the proteins of the silk after being secreted by glands. The post-translational modifications for example, enable a diversification of activities of the proteins encoded by all organisms to significantly increase proteome diversity and complexity. A structural characterization of proteins by a proteomics approach as that intended in this study might be performed using a combination of sophisticated technologies and sensitive subjects such as 2D-SDS-PAGE, high performance liquid chromatography and mass spectrometry. Thus, performing a chemical prospecting in a systematic way, we obtain information from the structural and chemical properties of silk proteins of Nephila clavipes spider web, allowing its development in biotechnological applications.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ARCURI, HELEN ANDRADE; PRIEWALDER, HELGA; SALLES, HELIANA CLARA; PALMA, MARIO SERGIO; LUBEC, GERT. Structural Model for the Spider Silk Protein Spidroin-1. JOURNAL OF PROTEOME RESEARCH, v. 14, n. 9, p. 3859-3870, SEP 2015. (10/19051-6, 13/26451-9, 11/51684-1)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; LAMPRECHT, GUENTHER; CHEN, WEI-QIANG; HEO, SEOK; HARDY, JOHN GEORGE; PRIEWALDER, HELGA; SCHEIBEL, THOMAS RAINER; PALMA, MARIO SERGIO; LUBEC, GERT. Structure and post-translational modifications of the web silk protein spidroin-1 from Nephila spiders. JOURNAL OF PROTEOMICS, v. 105, n. SI, p. 174-185, JUN 13 2014. (10/19051-6, 11/51684-1)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ARCURI, HELEN ANDRADE; ESTEVES, FRANCIELE GREGO; PALMA, MARIO SERGIO; LUBEC, GERT. Spider silk proteome provides insight into the structural characterization of Nephila clavipes flagelliform spidroin. SCIENTIFIC REPORTS, v. 8, OCT 2 2018. (16/16212-5, 10/19051-6, 13/26451-9, 11/51684-1, 15/14220-8)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ARCURI, HELEN ANDRADE; LUBEC, GERT; PALMA, MARIO SERGIO. Structural characterization of the major ampullate silk spidroin-2 protein produced by the spider Nephila clavipes. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1864, n. 10, p. 1444-1454, OCT 2016. (10/19051-6, 13/26451-9, 11/51684-1)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; CAVIQUIOLI GARCIA, ANA MARIA; ARCURI, HELEN ANDRADE; ESTEVES, FRANCIELE GREGO; SALLES, HELIANA CLARA; LUBEC, GERT; PALMA, MARIO SERGIO. Silkomics: Insight into the Silk Spinning Process of Spiders. JOURNAL OF PROTEOME RESEARCH, v. 15, n. 4, p. 1179-1193, APR 2016. (10/19051-6, 13/26451-9, 11/51684-1)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; LAMPRECHT, GUENTHER; CHEN, WEI-QIANG; HEO, SEOK; HARDY, JOHN GEORGE; PRIEWALDER, HELGA; SCHEIBEL, THOMAS RAINER; PALMA, MARIO SERGIO; LUBEC, GERT. Structure and post-translational modifications of the web silk protein spidroin-1 from Nephila spiders. JOURNAL OF PROTEOMICS, v. 105, p. 12-pg., 2014-06-13. (11/51684-1, 10/19051-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS-PINTO, José Roberto Aparecido dos. Análise estrutural das proteínas da seda da teia da aranha Nephila clavipes por uma abordagem proteômica. 2014. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.