New insights into the role of serum amyloid A (SAA) on obesity and insulin resistance

• Grant number: 10/18498-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2011
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Ana Campa
• Grantee: Edson Mendes de Oliveira
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The inflammation resulting from obesity is well characterized. More recently it has been discussed, if obesity could be a consequence of the inflammatory process. It is known that lipopolysaccharide (LPS), administered in very low doses by continuous infusion (mimicking the condition of endotoxemia caused by intestinal microbiota), induces insulin resistance, obesity and diabetes in mice. In this study we attempted to assess if acute inflammation and sleep restriction is capable of inducing obesity. One of the striking systemic events that occurs during an acute phase is the synthesis of the protein serum amyloid A (SAA). Our interest in SAA is derived from results obtained in our laboratory that show an action on proliferation, differentiation and lipolysis of pre-adipocytes 3T3-L1. In the applicant's master's thesis it was also observed that the expression of SAA is induced by hypoxia in pre-adipocytes and adipocytes. Thus, the current project proposes the induction of multiple acute inflammatory processes in mice C57BL6/J, caused by intraperitoneal administration of high doses of LPS. After the recovery period, morphological and metabolic analyses of adipose tissue will be made. Additional tests include the effect of serum from inflamed animals on the proliferation, differentiation and lipolysis of pre-adipocytes 3T3-L1 and in vivo analysis. The latter assay will be made implanting pre-adipocytes 3T3-L1 labeled with a fluorescent protein in mice subjected to an acute inflammation.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, EDSON M.; ASCAR, THAIS P.; SILVA, JACQUELINE C.; SANDRI, SILVANA; MIGLIORINI, SILENE; FOCK, RICARDO A.; CAMPA, ANA. Serum amyloid A links endotoxaemia to weight gain and insulin resistance in mice. Diabetologia, v. 59, n. 8, p. 1760-1768, AUG 2016. (10/18498-7, 11/24052-4)

DE OLIVEIRA, EDSON M.; VISNIAUSKAS, BRUNA; TUFIK, SERGIO; ANDERSEN, MONICA L.; CHAGAS, JAIR R.; CAMPA, ANA. Serum Amyloid A Production Is Triggered by Sleep Deprivation in Mice and Humans: Is That the Link between Sleep Loss and Associated Comorbidities?. NUTRIENTS, v. 9, n. 3, MAR 2017. (10/18498-7, 11/24052-4)

OKADA, SABRINA SAYORI; DE OLIVEIRA, EDSON MENDES; DE ARAUJO, TOMAZ HENRIQUE; RODRIGUES, MARIA RITA; ALBUQUERQUE, RENATA CHAVES; MORTARA, RENATO ARRUDA; TANIWAKI, NOEMI NOSOMI; NAKAYA, HELDER IMOTO; CAMPA, ANA; RAMOS MORENO, ANA CAROLINA. Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization. Cellular Immunology, v. 300, p. 18-25, FEB 2016. (09/14632-3, 10/18498-7)

DE OLIVEIRA, EDSON MENDES; SILVA, JACQUELINE C.; ASCAR, THAIS P.; SANDRI, SILVANA; MARCHI, ALEXANDRE F.; MIGLIORINI, SILENE; NAKAYA, HELDER T., I; FOCK, RICARDO A.; CAMPA, ANA. Acute Inflammation Is a Predisposing Factor for Weight Gain and Insulin Resistance. PHARMACEUTICS, v. 14, n. 3, p. 13-pg., 2022-03-01. (11/24052-4, 10/18498-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Edson Mendes de. New insights into the role of serum amyloid A (SAA) on obesity and insulin resistance. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.